In this study we have investigated the effects of a tumour suppressor microRNA, miR-214, on gene expression in HPV-positive (CaSki) and HPV-negative cervical cancer cells (C33A) by RNA sequencing using next generation sequencing. The HPV-positive and HPV-negative cervical cancer cells were either miR-214-knocked-out or miR-214-overexpressed. Gene expression analysis showed that a total of 904 genes were upregulated and 365 genes were downregulated between HPV-positive and HPV-negative cervical cancer cells with a fold change of ±2. Furthermore, 11 differentially expressed and relevant genes ( TNFAIP3, RAB25, MET, CYP1B1, NDRG1, CD24, LOXL2, CD44, PMS2, LATS1 and MDM1 ) which showed a fold change of ±5 were selected to confirm by real-time PCR. This study represents the first report of miR-214 on global gene expression in the context of HPV.

中文翻译：

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通过 CRISPR 敲除 miR-214 对 HPV 阳性和 HPV 阴性宫颈癌细胞的差异转录组分析

在这项研究中，我们通过使用下一代测序的 RNA 测序研究了肿瘤抑制因子 microRNA miR-214 对 HPV 阳性 (CaSki) 和 HPV 阴性宫颈癌细胞 (C33A) 中基因表达的影响。HPV 阳性和 HPV 阴性的宫颈癌细胞被 miR-214 敲除或过表达 miR-214。基因表达分析显示，HPV阳性和HPV阴性宫颈癌细胞共有904个基因上调，365个基因下调，倍数变化为±2。此外，选择显示±5倍变化的11个差异表达和相关基因（TNFAIP3、RAB2​​5、MET、CYP1B1、NDRG1、CD24、LOXL2、CD44、PMS2、LATS1和MDM1）以通过实时PCR确认。这项研究代表了 miR-214 在 HPV 背景下全球基因表达的第一份报告。