A novel marine actinomycete strain designated ICN19^T was isolated from the subtidal sediment of Chinnamuttam coast of Kanyakumari, India and subjected to polyphasic taxonomic analysis. Neighbour-joining tree based on 16S rRNA gene sequences of validly described type strains had revealed the strain ICN19^T formed distinct cluster with Streptomyces wuyuanensis CGMCC 4.7042^T, Streptomyces tirandamycinicus HNM0039^T and Streptomyces spongiicola HNM0071^T. Morphological, physiological and chemotaxonomic characteristics were consistent with those of members of the genus Streptomyces. The strain possessed ll-diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was identified as MK-9(H8) (70%), MK-9(H6) (20%) and MK-9(H2) (2%), with the major cellular fatty acids (>10%) being anteiso-C_15:0, C_16:0 and iso-C_16:0. The main polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides and three unidentified phospholipids. The dendrogram generated on the basis of MALDI-TOF mass spectra supports the strain differentiated from its neigbours. The genome sequence of strain ICN19^T was 9,010,366 bp in size with a total of 7420 protein-coding genes and 98 RNA genes. The genomic G+C content of the novel strain was 71.27 mol%. The DNA–DNA relatedness between strain ICN19^T and the reference strains with S. wuyuanensis CGMCC 4.7042^T, S. tirandamycinicus HNM0039^T and S. spongiicola HNM0071^T were 42.8%, 39.5% and 38%, respectively. Based on differences in physiological, biochemical, chemotaxonomic differences and whole-genome characteristics the isolated strain represents a novel species of the genus Streptomyces, for which the name Streptomyces marianii sp. nov. is proposed. Type strain is ICN19^T (=MCC 3599^T = KCTC 39749^T).

从印度 Ka​​nyakumari 的 Chinnamuttam 海岸潮下沉积物中分离出一种名为 ICN19 ^T的新型海洋放线菌菌株，并进行了多相分类分析。基于有效描述的典型菌株的16S rRNA基因序列的邻接树显示，菌株ICN19 ^T与Streptomyces wuyuanensis CGMCC 4.7042 ^T 、 Streptomyces tirandamycinicus HNM0039 ^T和Streptomyces spongiicola HNM0071 ^T形成不同的簇。形态、生理和化学分类学特征与链霉菌属成员一致。该菌株具有11-二氨基庚二酸作为诊断二氨基酸。主要的类异戊二烯醌被鉴定为 MK-9(H8) (70%)、MK-9(H6) (20%) 和 MK-9(H2) (2%)，主要细胞脂肪酸 (>10%) ) 是anteiso -C _15:0 、C _16:0和iso -C _16:0 。发现主要的极性脂质是二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇甘露糖苷和三种未鉴定的磷脂。基于 MALDI-TOF 质谱生成的树状图支持与相邻应变不同的应变。 ICN19 ^T菌株基因组序列全长9,010,366 bp，共有7420个蛋白质编码基因和98个RNA基因。新菌株基因组G+C含量为71.27 mol%。菌株ICN19 ^T与参考菌株S. wuyuanensis CGMCC 4.7042 ^T 、 S. tirandamycinicus HNM0039 ^T和S. wuyuanensis CGMCC 4.7042 T之间的DNA-DNA相关性。 海绵菌 HNM0071 ^T分别为 42.8%、39.5% 和 38%。基于生理、生化、化学分类学差异和全基因组特征的差异，分离的菌株代表了链霉菌属的新物种，其名称为Streptomyces marianii sp。十一月被提议。典型菌株是 ICN19 ^T (=MCC 3599 ^T = KCTC 39749 ^T )。