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Streptomyces marianii sp. nov., a novel marine actinomycete from southern coast of India.
The Journal of Antibiotics ( IF 2.1 ) Pub Date : 2020-08-13 , DOI: 10.1038/s41429-020-0360-z
Appadurai Muthamil Iniyan 1, 2 , Joachim Wink 2 , Wiebke Landwehr 2 , Eedara Veera Venkata Ramprasad 3 , Chintalapati Sasikala 3 , Chintalapati Venkata Ramana 4 , Peter Schumann 5 , Cathrin Spröer 5 , Boyke Bunk 5 , Francis-Joseph Rosemary Sharmila Joseph 1 , Sam Albert Joshua 1 , Eby-Nesar Stella-Glory Deepak Shyl 1 , Rajaretinam Rajesh Kannan 6 , Samuel Gnana Prakash Vincent 1
Affiliation  

A novel marine actinomycete strain designated ICN19T was isolated from the subtidal sediment of Chinnamuttam coast of Kanyakumari, India and subjected to polyphasic taxonomic analysis. Neighbour-joining tree based on 16S rRNA gene sequences of validly described type strains had revealed the strain ICN19T formed distinct cluster with Streptomyces wuyuanensis CGMCC 4.7042T, Streptomyces tirandamycinicus HNM0039T and Streptomyces spongiicola HNM0071T. Morphological, physiological and chemotaxonomic characteristics were consistent with those of members of the genus Streptomyces. The strain possessed ll-diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was identified as MK-9(H8) (70%), MK-9(H6) (20%) and MK-9(H2) (2%), with the major cellular fatty acids (>10%) being anteiso-C15:0, C16:0 and iso-C16:0. The main polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides and three unidentified phospholipids. The dendrogram generated on the basis of MALDI-TOF mass spectra supports the strain differentiated from its neigbours. The genome sequence of strain ICN19T was 9,010,366 bp in size with a total of 7420 protein-coding genes and 98 RNA genes. The genomic G+C content of the novel strain was 71.27 mol%. The DNA–DNA relatedness between strain ICN19T and the reference strains with S. wuyuanensis CGMCC 4.7042T, S. tirandamycinicus HNM0039T and S. spongiicola HNM0071T were 42.8%, 39.5% and 38%, respectively. Based on differences in physiological, biochemical, chemotaxonomic differences and whole-genome characteristics the isolated strain represents a novel species of the genus Streptomyces, for which the name Streptomyces marianii sp. nov. is proposed. Type strain is ICN19T (=MCC 3599T = KCTC 39749T).



中文翻译:


马里亚尼链霉菌 (Streptomyces marianii sp.) 11月,一种来自印度南部海岸的新型海洋放线菌。



从印度 Ka​​nyakumari 的 Chinnamuttam 海岸潮下沉积物中分离出一种名为 ICN19 T的新型海洋放线菌菌株,并进行了多相分类分析。基于有效描述的典型菌株的16S rRNA基因序列的邻接树显示,菌株ICN19 TStreptomyces wuyuanensis CGMCC 4.7042 TStreptomyces tirandamycinicus HNM0039 TStreptomyces spongiicola HNM0071 T形成不同的簇。形态、生理和化学分类学特征与链霉菌属成员一致。该菌株具有11-二氨基庚二酸作为诊断二氨基酸。主要的类异戊二烯醌被鉴定为 MK-9(H8) (70%)、MK-9(H6) (20%) 和 MK-9(H2) (2%),主要细胞脂肪酸 (>10%) ) 是anteiso -C 15:0 、C 16:0iso -C 16:0 。发现主要的极性脂质是二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇甘露糖苷和三种未鉴定的磷脂。基于 MALDI-TOF 质谱生成的树状图支持与相邻应变不同的应变。 ICN19 T菌株基因组序列全长9,010,366 bp,共有7420个蛋白质编码基因和98个RNA基因。新菌株基因组G+C含量为71.27 mol%。菌株ICN19 T与参考菌株S. wuyuanensis CGMCC 4.7042 TS. tirandamycinicus HNM0039 TS. wuyuanensis CGMCC 4.7042 T之间的DNA-DNA相关性。 海绵菌 HNM0071 T分别为 42.8%、39.5% 和 38%。基于生理、生化、化学分类学差异和全基因组特征的差异,分离的菌株代表了链霉菌属的新物种,其名称为Streptomyces marianii sp。十一月被提议。典型菌株是 ICN19 T (=MCC 3599 T = KCTC 39749 T )。

更新日期:2020-08-14
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