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Streptomyces marianii sp. nov., a novel marine actinomycete from southern coast of India

The Journal of Antibiotics volume 74, pages 59–69 (2021)Cite this article

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Abstract

A novel marine actinomycete strain designated ICN19T was isolated from the subtidal sediment of Chinnamuttam coast of Kanyakumari, India and subjected to polyphasic taxonomic analysis. Neighbour-joining tree based on 16S rRNA gene sequences of validly described type strains had revealed the strain ICN19T formed distinct cluster with Streptomyces wuyuanensis CGMCC 4.7042T, Streptomyces tirandamycinicus HNM0039T and Streptomyces spongiicola HNM0071T. Morphological, physiological and chemotaxonomic characteristics were consistent with those of members of the genus Streptomyces. The strain possessed ll-diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was identified as MK-9(H8) (70%), MK-9(H6) (20%) and MK-9(H2) (2%), with the major cellular fatty acids (>10%) being anteiso-C15:0, C16:0 and iso-C16:0. The main polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides and three unidentified phospholipids. The dendrogram generated on the basis of MALDI-TOF mass spectra supports the strain differentiated from its neigbours. The genome sequence of strain ICN19T was 9,010,366 bp in size with a total of 7420 protein-coding genes and 98 RNA genes. The genomic G+C content of the novel strain was 71.27 mol%. The DNA–DNA relatedness between strain ICN19T and the reference strains with S. wuyuanensis CGMCC 4.7042T, S. tirandamycinicus HNM0039T and S. spongiicola HNM0071T were 42.8%, 39.5% and 38%, respectively. Based on differences in physiological, biochemical, chemotaxonomic differences and whole-genome characteristics the isolated strain represents a novel species of the genus Streptomyces, for which the name Streptomyces marianii sp. nov. is proposed. Type strain is ICN19T (=MCC 3599T = KCTC 39749T).

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Acknowledgements

The support of the Department of Science and Technology, Science and Engineering Research Board, Govt. of India [F.No. SR/SO/HS-104/2012] to SGPV is gratefully acknowledged. We thank Prof. Peter Kampfer, University of Gießen, Germany for his help on SEM analysis. We thank to Prof. Aharon Oren, The Hebrew University of Jerusalem, Israel for his expert suggestion for correct species epithet and Latin etymology. We thank Romy Schade, Simone Severitt and Carola Berg for excellent technical assistance.

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Authors and Affiliations

  1. Centre for Marine Science and Technology (CMST), Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari District, Tamil Nadu, 629502, India

    Appadurai Muthamil Iniyan, Francis-Joseph Rosemary Sharmila Joseph, Sam Albert Joshua, Eby-Nesar Stella-Glory Deepak Shyl & Samuel Gnana Prakash Vincent

  2. Microbial Strain Collection, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, Braunschweig, D-38124, Germany

    Appadurai Muthamil Iniyan, Joachim Wink & Wiebke Landwehr

  3. Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J.N.T. University Hyderabad, Kukatpally, Hyderabad, 500085, India

    Eedara Veera Venkata Ramprasad & Chintalapati Sasikala

  4. Bacterial Discovery and Metabolomics Laboratory, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, PO Central University, Hyderabad, 500046, India

    Chintalapati Venkata Ramana

  5. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstraße 7b, D-38124, Braunschweig, Germany

    Peter Schumann, Cathrin Spröer & Boyke Bunk

  6. Infectious Disease Biology Lab, Centre for Molecular and Nanomedical Sciences, Centre for Nanoscience and Nanotechnology, School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Jeppiaar Nagar, Chennai, Tamil Nadu, 600119, India

    Rajaretinam Rajesh Kannan

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Iniyan, A.M., Wink, J., Landwehr, W. et al. Streptomyces marianii sp. nov., a novel marine actinomycete from southern coast of India. J Antibiot 74, 59–69 (2021). https://doi.org/10.1038/s41429-020-0360-z

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