In this study, an avian leukosis virus (ALV) strain (GX-2020-01) was isolated from a three-yellow chicken, and its complete genome was 7570 bp long with the typical organization “5’LTR-gag-pol-env-3’LTR.” Phylogenetic analysis and sequence comparison revealed that it belongs to the ALV-J subgroup. However, the LTR region of GX-2020-01 is highly similar to that of reference strains of ALV-K/E (96.61%-97.10%), demonstrating that this novel isolate is a natural recombinant. The replication efficiency of GX-2020-01 was significantly lower than the previously isolated ALV-J strain (NX0101), indicating that the recombination event might have resulted in slower virus replication, making it harder for it to be detected through routine testing.

在这项研究中，从三只黄色的鸡中分离出禽白血病病毒（ALV）株（GX-2020-01），其完整基因组长7570 bp，典型组织为“ 5'LTR- gag - pol - env -3'LTR。” 系统发育分析和序列比较表明，它属于ALV-J亚组。但是，GX-2020-01的LTR区域与ALV-K / E参考菌株的LTR区域高度相似（96.61％-97.10％），表明该新型分离株是天然重组体。GX-2020-01的复制效率显着低于以前分离的ALV-J菌株（NX0101），表明重组事件可能导致病毒复制较慢，从而使其难以通过常规测试检测到。