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Complete genome sequence of a novel recombinant avian leukosis virus isolated from a three-yellow chicken

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Abstract

In this study, an avian leukosis virus (ALV) strain (GX-2020-01) was isolated from a three-yellow chicken, and its complete genome was 7570 bp long with the typical organization “5’LTR-gag-pol-env-3’LTR.” Phylogenetic analysis and sequence comparison revealed that it belongs to the ALV-J subgroup. However, the LTR region of GX-2020-01 is highly similar to that of reference strains of ALV-K/E (96.61%-97.10%), demonstrating that this novel isolate is a natural recombinant. The replication efficiency of GX-2020-01 was significantly lower than the previously isolated ALV-J strain (NX0101), indicating that the recombination event might have resulted in slower virus replication, making it harder for it to be detected through routine testing.

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Funding

This study was supported by the National Key Research and Development Program of China [grant number 2017YFF0211103].

Author information

Authors and Affiliations

  1. Technical Center of Qingdao Customs, NO. 70 Qutangxia Road, Qingdao, 266002, Shandong, China

    Tao Sun, Wei Han, Weili Yin & Baohai Fang

  2. Department of Breast Surgery, Qilu Hospital (Qingdao) of Shandong University, No. 758 Hefei Road, Shibei District, Qingdao, 266000, Shandong, China

    Ximei Wang

  3. Nanning Mootek Intelligent Technology Co., Ltd, Yongfu Road, Yongning District, Nanning, 530000, Guangxi, China

    Xiao Lin

  4. China Animal Health and Epidemiology Center, Qingdao, 266000, China

    Yang Li

  5. Shanghai Pharmaceutical Group Qingdao Guofeng Pharmaceutical Co. LTD, NO.18 Songhuajiang Road, Economic and Technological Development Area, Qingdao, 266000, China

    Xiaoling Ma

Authors
  1. Tao Sun
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  2. Ximei Wang
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  3. Wei Han
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  4. Xiaoling Ma
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  5. Weili Yin
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  6. Baohai Fang
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  7. Xiao Lin
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  8. Yang Li
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Corresponding authors

Correspondence to Xiao Lin or Yang Li.

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Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Ethical approval

All the experimental animals in this study were cared for and maintained throughout the experiments strictly following the ethics and biosecurity guidelines approved by the Institutional Animal Care and Use Committee of Shandong.

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Handling Editor: William G. Dundon.

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705_2020_4764_MOESM1_ESM.jpg

Fig. S1 Comparison of the viral replication dynamics of GX-2020-01 and NX0101. Growth curves were generated by measuring the viral titers at various time points. The viral titers were determined using samples harvested each day and were expressed as TCID50 mL–1. The mean ± SD values from three independent experiments are shown (JPG 933 kb)

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Sun, T., Wang, X., Han, W. et al. Complete genome sequence of a novel recombinant avian leukosis virus isolated from a three-yellow chicken. Arch Virol 165, 2615–2618 (2020). https://doi.org/10.1007/s00705-020-04764-7

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  • DOI: https://doi.org/10.1007/s00705-020-04764-7

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