The genome sequence of a mitovirus found in an isolate of Diaporthe rudis, one of the causal agents of Phomopsis dieback on grapevines, was determined by two high-throughput sequencing approaches, small RNA and total RNA sequencing. The genome of this mitovirus is 2,455 nt in length and includes a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp). A BLASTx comparison of the full-length genome sequence showed the highest similarity (54.15%) with that of Colletotrichum falcatum mitovirus 1 (CfMV1). Our results reveal a new member of the genus Mitovirus first detected in D. rudis (Fr.) Nitschke, with the proposed name “Diaporthe rudis mitovirus 1” (DrMV1).

通过两种高通量测序方法（小RNA和总RNA测序）确定了在金丝桃（Diaporthe rudis）的分离株中发现的线粒体病毒的基因组序列，该分离物是葡萄的枯萎病菌的致病因子之一。该线粒体病毒的基因组长度为2455 nt，并包括一个编码RNA依赖性RNA聚合酶（RdRp）的单个大开放阅读框（ORF）。全长基因组序列的BLASTx比较显示与金刚Colletotrichum falcatum线粒体病毒1（CfMV1）的相似性最高（54.15％）。我们的研究结果揭示了线粒体病毒属的一个新成员，该成员首次在尼氏小球藻（D. rudis（Fr.）Nitschke中被检测到，并被命名为“ Diaporthe rudis mitovirus 1”（DrMV1）。