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A novel mitovirus detected in Diaporthe rudis, a fungus associated with Phomopsis dieback on grapevines

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Abstract

The genome sequence of a mitovirus found in an isolate of Diaporthe rudis, one of the causal agents of Phomopsis dieback on grapevines, was determined by two high-throughput sequencing approaches, small RNA and total RNA sequencing. The genome of this mitovirus is 2,455 nt in length and includes a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp). A BLASTx comparison of the full-length genome sequence showed the highest similarity (54.15%) with that of Colletotrichum falcatum mitovirus 1 (CfMV1). Our results reveal a new member of the genus Mitovirus first detected in D. rudis (Fr.) Nitschke, with the proposed name “Diaporthe rudis mitovirus 1” (DrMV1).

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Acknowledgements

We are grateful to Milica Raco, Eliška Peňázová and Jakub Pečenka for their help in the laboratory work and data processing, and Tomáš Kiss for English editing. This research was supported by the project Phytophthora Research Centre Reg. No. CZ.02.1.01/0.0/0.0/15_003/0000453, which was co-financed by the European Regional Development Fund and by the Internal Grant Agency of MENDELU (IGA-ZF/2020-DP008).

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Authors and Affiliations

  1. Mendeleum-Institute of Genetics, Faculty of Horticulture, Mendel University in Brno, Valticka 334, 691 44, Lednice, Czech Republic

    Mária Kocanová & Aleš Eichmeier

  2. Phytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic

    Leticia Botella

  3. Biotechnological Centre, Faculty of Agriculture, University of South Bohemia, Na Sadkach 1780, 37005, Ceske Budejovice, Czech Republic

    Leticia Botella

Authors
  1. Mária Kocanová
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  2. Aleš Eichmeier
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  3. Leticia Botella
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Correspondence to Mária Kocanová.

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Kocanová, M., Eichmeier, A. & Botella, L. A novel mitovirus detected in Diaporthe rudis, a fungus associated with Phomopsis dieback on grapevines. Arch Virol 165, 2405–2408 (2020). https://doi.org/10.1007/s00705-020-04755-8

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  • DOI: https://doi.org/10.1007/s00705-020-04755-8

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