Tuber species may be regarded as complex microhabitats hosting diverse microorganisms inside their fruiting bodies. Here, we investigated the structure of microbial communities inhabiting the gleba of wild growing (in stands) T. aestivum, using Illumina sequencing and culture-based methods. The two methods used in combination allowed to extract more information on complex microbiota of Tuber aestivum gleba. Analysis of the V3–V4 region of 16S rDNA identified nine phyla of bacteria present in the gleba of T. aestivum ascomata, mostly Proteobacteria from the family Bradyrhizobiaceae. Our results ideally match the earlier data for other Tuber species where the family Bradyrhizobiaceae was the most represented. The ITS1 region of fungal rDNA represented six alien fungal species belonging to three phyla. To complement the metagenomic analysis, cultivable fungi and bacteria were obtained from the gleba of the same T. aestivum fruiting bodies. The identified fungi mostly belong to the phylum Basidiomycota and same to Ascomycota. Analysis of cultivable bacteria revealed that all the specimens were colonized by different strains of Bacillus. Fungal community inhabiting T. aestivum fruiting bodies was never shown before.

中文翻译：

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勃艮第松露（Tuber aestivum Vittad.）子实体中细菌和真菌的鉴定


块茎物种可被视为其子实体内承载着多种微生物的复杂微生境。在这里，我们使用 Illumina 测序和基于培养的方法研究了栖息在野生生长（立地）普通小麦 (T. aestivum) 的 gleba 中的微生物群落的结构。结合使用两种方法可以提取有关普通小麦复杂微生物群的更多信息。对 16S rDNA V3-V4 区域的分析鉴定出存在于 T. aestivum ascomata 的 gleba 中的九个细菌门，主要是来自慢生根瘤菌科 (Bradyrhizobiaceae) 的变形菌。我们的结果与其他块茎物种的早期数据完美匹配，其中以慢生根瘤菌科（Bradyrhizobiaceae）最具代表性。真菌rDNA的ITS1区域代表了属于三个门的六种外来真菌。为了补充宏基因组分析，从相同的普通小麦子实体的 gleba 中获得了可培养的真菌和细菌。已鉴定的真菌大多属于担子菌门和子囊菌门。对可培养细菌的分析表明，所有样本都被不同的芽孢杆菌菌株定殖。以前从未显示过栖息在普通小麦子实体中的真菌群落。