Purpose To investigate the effect of Ag-1031 on apoptosis of gastric cancer AGS cells and the PI3K/AKT/mTOR signaling pathway. Methods Gastric cancer cells were treated with different doses of Ag-1031. Cell viability was determined with MTT assay. Apoptosis was analyzed with Hoechst 33342 staining and Annexin V-FITC/PI double staining. The protein expression levels of p-PI3K, p-AKT, p-mTOR, PI3K, AKT and mTOR were assayed with western blotting method. Results Different doses of Ag-1031 significantly inhibited the proliferation of AGS cells. Moreover, Ag-1031 induced apoptosis,in gastric cancer cells. In addition, Ag-1031 significantly and dose-dependently inhibited the protein expressions of p-PI3K, p-AKT and p-mTOR. However, it had no effect on the protein expressions of PI3K, AKT and mTOR. Conclusion In this study, it was found for the first time that Ag-1031 exerts an anti-tumor effect by induction apoptosis in gastric cancer AGS cells, through a mechanism related to inhibition of the activation of the PI3K/AKT/mTOR signaling pathway.

中文翻译：

---

Ag-1031对胃癌AGS细胞凋亡的影响及其对PI3K/AKT/mTOR信号通路的影响

目的探讨Ag-1031对胃癌AGS细胞凋亡及PI3K/AKT/mTOR信号通路的影响。方法用不同剂量的Ag-1031处理胃癌细胞。用MTT测定法测定细胞活力。用 Hoechst 33342 染色和 Annexin V-FITC/PI 双染色分析细胞凋亡。用蛋白质印迹法检测p-PI3K、p-AKT、p-mTOR、PI3K、AKT和mTOR的蛋白表达水平。结果不同剂量的Ag-1031均能显着抑制AGS细胞的增殖。此外，Ag-1031 诱导胃癌细胞凋亡。此外，Ag-1031 显着且剂量依赖性地抑制 p-PI3K、p-AKT 和 p-mTOR 的蛋白质表达。然而，它对PI3K、AKT和mTOR的蛋白表达没有影响。结论 在本研究中，