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Effect of Ag-1031 on apoptosis in gastric cancer AGS cells and its effects on the PI3K/AKT/mTOR signaling pathway

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Abstract

Purpose

To investigate the effect of Ag-1031 on apoptosis of gastric cancer AGS cells and the PI3K/AKT/mTOR signaling pathway.

Methods

Gastric cancer cells were treated with different doses of Ag-1031. Cell viability was determined with MTT assay. Apoptosis was analyzed with Hoechst 33342 staining and Annexin V-FITC/PI double staining. The protein expression levels of p-PI3K, p-AKT, p-mTOR, PI3K, AKT and mTOR were assayed with western blotting method.

Results

Different doses of Ag-1031 significantly inhibited the proliferation of AGS cells. Moreover, Ag-1031 induced apoptosis,in gastric cancer cells. In addition, Ag-1031 significantly and dose-dependently inhibited the protein expressions of p-PI3K, p-AKT and p-mTOR. However, it had no effect on the protein expressions of PI3K, AKT and mTOR.

Conclusion

In this study, it was found for the first time that Ag-1031 exerts an anti-tumor effect by induction apoptosis in gastric cancer AGS cells, through a mechanism related to inhibition of the activation of the PI3K/AKT/mTOR signaling pathway.

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Acknowledgements

The work was supported by Provincial Quality Engineering Project of colleges and universities in Anhui Province (2019xfxm90).

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Authors and Affiliations

  1. Department of Hepatobiliary Surgery, The Second People’s Hospital of Wuhu, No. 259, Jiuhua Middle Road, Wuhu, 241001, Anhui, China

    Jianhua Dai, Danfeng Liu, Longjiang Chen & LiXia Sun

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  1. Jianhua Dai
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  2. Danfeng Liu
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  3. Longjiang Chen
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  4. LiXia Sun
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Contributions

This study was done by the authors named in this article and the authors accept all liabilities resulting from claims which relate to this article and its contents. The study was conceived and designed by JD; DL, LC, LS collected and analyzed the data. JD wrote the manuscript. All authors read and approved the manuscript prior to publication.

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Correspondence to LiXia Sun.

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Dai, J., Liu, D., Chen, L. et al. Effect of Ag-1031 on apoptosis in gastric cancer AGS cells and its effects on the PI3K/AKT/mTOR signaling pathway. Biotechnol Lett 42, 2447–2452 (2020). https://doi.org/10.1007/s10529-020-02954-6

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  • DOI: https://doi.org/10.1007/s10529-020-02954-6

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