Photodynamic therapy (PDT) is one of the major therapeutic methods for the treatment of infectious diseases and cancer. Recently, cell culture has been used to determine the effect of a given substance on various pathological conditions, such as cancer. In this study, we aimed to investigate the effect of a Zn- phthalocyanine (ZnPc) derivative on selected cancer cells via a cell culture medium. Methylthiazole tetrazolium (MTT) assay was applied to evaluate the cytotoxic activity of 2(3),9(10),16(17),23(24)–tetrakis-(6-methylpyridin-2-yloxy)phthalocyaninato Zn(II) on rat glioma cells (C6 glioma), human lung cancer cells (H1299) and human umbilical vein endothelial cells (HUVEC). The levels of the lipid peroxidation were determined by measuring the amount of the thiobarbituric acid reactive substance (TBARS) produced using the TBARS assay. The relationship between the oxidative damage and the effective concentration of cytotoxic ZnPc was determined from the results. The apoptotic and genotoxic effects of the phthalocyanine (Pc) were also investigated. Density functional/time-dependent density functional theory (DFT/TD-DFT) methods were used to determine the molecular excited state properties of the ZnPc and chloroaluminum phthalocyanine (ClAlPc) previously reported by Castilho-Fernandes et al. The computed and experimental data were used to establish a link between the electronic and anticancer properties of the Pcs.

中文翻译：

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2(3),9(10),16(17),23(24)-Tetrakis-(6-Methylpyridin-2) 的抗癌活性研究和密度泛函/时间依赖性密度泛函理论 (DFT/TD-DFT) 计算-Yloxy)Phthalocyaninato Zn(II)。

光动力疗法（PDT）是治疗传染病和癌症的主要治疗方法之一。最近，细胞培养已被用于确定给定物质对各种病理状况（例如癌症）的影响。在这项研究中，我们旨在通过细胞培养基研究锌酞菁 (ZnPc) 衍生物对选定癌细胞的影响。应用甲基噻唑四唑 (MTT) 测定法评估 2(3),9(10),16(17),23(24)–四-(6-甲基吡啶-2-基氧基)酞菁锌 (II) 的细胞毒活性大鼠胶质瘤细胞 (C6 胶质瘤)、人肺癌细胞 (H1299) 和人脐静脉内皮细胞 (HUVEC)。通过测量使用 TBARS 测定法产生的硫代巴比妥酸反应物质 (TBARS) 的量来确定脂质过氧化的水平。从结果确定了氧化损伤与细胞毒性 ZnPc 有效浓度之间的关系。还研究了酞菁 (Pc) 的细胞凋亡和遗传毒性作用。密度泛函/时间相关密度泛函理论 (DFT/TD-DFT) 方法用于确定 Castilho-Fernandes 等人先前报道的 ZnPc 和氯铝酞菁 (ClAlPc) 的分子激发态特性。计算和实验数据用于建立 Pc 的电子和抗癌特性之间的联系。密度泛函/时间相关密度泛函理论 (DFT/TD-DFT) 方法用于确定 Castilho-Fernandes 等人先前报道的 ZnPc 和氯铝酞菁 (ClAlPc) 的分子激发态特性。计算和实验数据用于建立 Pc 的电子和抗癌特性之间的联系。密度泛函/时间相关密度泛函理论 (DFT/TD-DFT) 方法用于确定 Castilho-Fernandes 等人先前报道的 ZnPc 和氯铝酞菁 (ClAlPc) 的分子激发态特性。计算和实验数据用于建立 Pc 的电子和抗癌特性之间的联系。