The abnormal expression of circular RNA (circRNA) is bound up with the progress of various human cancers. This study aimed to reveal the potential role and mechanism of circBC048201 in the proliferation, migration, and invasion of bladder cancer cells. Quantitative real-time PCR was performed to detect the expression of circBC048201. Cell Counting Kit-8, colony formation, and transwell migration and invasion assays were used to confirm the in vitro functions of circBC048201. Western blot, RNA pull-down, and dual-luciferase reporter gene experiments were performed to study the potential mechanism. circBC048201 was abnormally highly expressed in bladder cancer tissues and cells, and the interference with circBC048201 inhibited bladder cancer cell proliferation, migration, and invasion. From the potential mechanism analysis, our data suggested that circBC048201 and miR-1184, miR-1184 and ITGA3 could bind to each other, and the interference with circBC048201 repressed bladder cancer cell proliferation, migration, and invasion through the miR-1184/ITGA3 axis. In summary, our results showed that circBC048201 was abnormally highly expressed in bladder cancer tissues and cells, and the interference with circBC048201 inhibited the proliferation, migration, and invasion of bladder cancer cells through the miR-1184/ITGA3 axis.

环状RNA（circRNA）的异常表达与各种人类癌症的进展密切相关。这项研究旨在揭示circBC048201在膀胱癌细胞的增殖，迁移和侵袭中的潜在作用和机制。进行定量实时PCR以检测circBC048201的表达。使用Cell Counting Kit-8，集落形成以及transwell迁移和侵袭实验来确认circBC048201的体外功能。进行了蛋白质印迹，RNA下拉和双荧光素酶报告基因实验以研究潜在的机制。circBC048201在膀胱癌组织和细胞中异常高表达，对circBC048201的干扰抑制了膀胱癌细胞的增殖，迁移和侵袭。从潜在的机制分析，我们的数据表明，circBC048201和miR-1184，miR-1184和ITGA3可以相互结合，并且对circBC048201的干扰抑制了miR-1184 / ITGA3轴对膀胱癌细胞的增殖，迁移和侵袭。总而言之，我们的结果表明，circBC048201在膀胱癌组织和细胞中异常高表达，并且对circBC048201的干扰抑制了miR-1184 / ITGA3轴对膀胱癌细胞的增殖，迁移和侵袭。