Abstract
The abnormal expression of circular RNA (circRNA) is bound up with the progress of various human cancers. This study aimed to reveal the potential role and mechanism of circBC048201 in the proliferation, migration, and invasion of bladder cancer cells. Quantitative real-time PCR was performed to detect the expression of circBC048201. Cell Counting Kit-8, colony formation, and transwell migration and invasion assays were used to confirm the in vitro functions of circBC048201. Western blot, RNA pull-down, and dual-luciferase reporter gene experiments were performed to study the potential mechanism. circBC048201 was abnormally highly expressed in bladder cancer tissues and cells, and the interference with circBC048201 inhibited bladder cancer cell proliferation, migration, and invasion. From the potential mechanism analysis, our data suggested that circBC048201 and miR-1184, miR-1184 and ITGA3 could bind to each other, and the interference with circBC048201 repressed bladder cancer cell proliferation, migration, and invasion through the miR-1184/ITGA3 axis. In summary, our results showed that circBC048201 was abnormally highly expressed in bladder cancer tissues and cells, and the interference with circBC048201 inhibited the proliferation, migration, and invasion of bladder cancer cells through the miR-1184/ITGA3 axis.
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This work was supported by the Zhejiang Provincial Medicine and Health Technology Project (2015KYA083).
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PJ designed this manuscript, performed the experiments, and was the major contributor in writing the manuscript. YZ participated in experiments and assisted in writing the manuscript. ZJX collected data and performed the statistical analysis. XYC participated in the revision of this manuscript. LPX made substantial contributions to the conception, design, and critical revision of the manuscript. All authors read and approved the final manuscript.
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11010_2020_3835_MOESM1_ESM.tif
The expressions of circBC048201 \( (2^{{ - \Delta C_{{\text{t}}} }} ) \) and miR-1184 \( (2^{{ - \Delta C_{{\text{t}}} }} ) \) in bladder cancer cells. a Detection of circBC048201 \( (2^{{ - \Delta C_{{\text{t}}} }} ) \). a Detection of miR-1184 \( (2^{{ - \Delta C_{{\text{t}}} }} ) \). ***P < 0.001 vs. SV-HUC-1 group. Supplementary file1 (TIF 107 kb)
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Jiang, P., Zhu, Y., Xu, Z. et al. Interference with circBC048201 inhibits the proliferation, migration, and invasion of bladder cancer cells through the miR-1184/ITGA3 axis. Mol Cell Biochem 474, 83–94 (2020). https://doi.org/10.1007/s11010-020-03835-2
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DOI: https://doi.org/10.1007/s11010-020-03835-2