Liquor from pretreatment of sugarcane bagasse is a potential substrate for multiple purposes due to the high concentration of residual sugars. Nevertheless, several potentially toxic byproducts are also present. However, a few microorganisms are able to overcome this toxicity by growing on these liquors. Twenty-five filamentous fungi were evaluated in submerged cultivation, but none was able to grow using liquor at a concentration of 100% as the liquid medium. However, six fungi were selected for enzyme induction after being grown in diluted liquor at 50% (v/v) using two feed pulses. Induction experiments were performed using 1% untreated and pretreated sugarcane bagasse. FPase and xylanase activities were detected for all six fungi in submerged cultivation, whereas β-glucosidase was observed in four fungi. The highest xylanase activity (28.8 IU mL−1) was at 72 h for T. harzianum P49P11 using pretreated-SCB as an inducer. This work showed a successful alternative for the final destination of liquor residue as substrate for fungi cultivation prior to enzyme production.

中文翻译：

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使用甘蔗渣和富含木糖的液体作为底物诱导真菌纤维素分解酶

由于高浓度的残糖，来自甘蔗渣预处理的酒是多种用途的潜在底物。然而，也存在几种潜在的有毒副产品。然而，一些微生物能够通过在这些液体上生长来克服这种毒性。对 25 种丝状真菌进行了沉水培养评估，但没有一种能够使用 100% 浓度的液体作为液体培养基生长。然而，在使用两个进料脉冲以 50% (v/v) 的稀释液中生长后，选择了六种真菌进行酶诱导。使用 1% 未处理和预处理的甘蔗渣进行诱导实验。在水下培养的所有六种真菌中都检测到了 FPase 和木聚糖酶活性，而在四种真菌中观察到了 β-葡萄糖苷酶。最高的木聚糖酶活性 (28. 使用预处理的 SCB 作为诱导剂，哈茨木霉 P49P11 的 8 IU mL-1) 在 72 小时。这项工作展示了一种成功的替代方案，可以在酶生产之前将酒渣作为真菌培养的底物。