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Induction of fungal cellulolytic enzymes using sugarcane bagasse and xylose-rich liquor as substrates

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Abstract

Liquor from pretreatment of sugarcane bagasse is a potential substrate for multiple purposes due to the high concentration of residual sugars. Nevertheless, several potentially toxic byproducts are also present. However, a few microorganisms are able to overcome this toxicity by growing on these liquors. Twenty-five filamentous fungi were evaluated in submerged cultivation, but none was able to grow using liquor at a concentration of 100% as the liquid medium. However, six fungi were selected for enzyme induction after being grown in diluted liquor at 50% (v/v) using two feed pulses. Induction experiments were performed using 1% untreated and pretreated sugarcane bagasse. FPase and xylanase activities were detected for all six fungi in submerged cultivation, whereas β-glucosidase was observed in four fungi. The highest xylanase activity (28.8 IU mL−1) was at 72 h for T. harzianum P49P11 using pretreated-SCB as an inducer. This work showed a successful alternative for the final destination of liquor residue as substrate for fungi cultivation prior to enzyme production.

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Acknowledgements

The authors would like to thank the technical staff of the National Laboratory of Science and Technology of Bioethanol (CTBE) and acknowledge the financial support provided by the Brazilian agency CAPES (finance code 001).

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Authors and Affiliations

  1. Laboratory of Biochemistry and Applied Microbiology, São Paulo State University-UNESP, IBILCE, 2265 Cristóvão Colombo, São José Do Rio Preto, São Paulo, 15054-000, Brazil

    Sidnei Emilio Bordignon, Olavo Perrone, Maurício Boscolo, Eleni Gomes & Roberto da Silva

  2. Brazilian Bioethanol Science and Technology Laboratory, CTBE, Polo II de Alta Tecnologia, 10000 Giuseppe Máximo Scolfaro, Campinas, SP, 13083-100, Brazil

    Priscila da Silva Delabona, Deise Lima & José Geraldo da Cruz Pradella

  3. Laboratory of Systematic Investigation in Biotechnology and Molecular Biodiversity, Federal University of Pará (UFPA), Augusto Correa, Belém, PA, 66075-110, Brazil

    Márcia Gleice da Silva Souza & Alberdan Silva Santos

Authors
  1. Sidnei Emilio Bordignon
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  2. Priscila da Silva Delabona
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  3. Deise Lima
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  4. Olavo Perrone
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  5. Márcia Gleice da Silva Souza
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  6. Alberdan Silva Santos
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  7. José Geraldo da Cruz Pradella
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  8. Maurício Boscolo
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  9. Eleni Gomes
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  10. Roberto da Silva
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Correspondence to Sidnei Emilio Bordignon.

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Bordignon, S.E., da Silva Delabona, P., Lima, D. et al. Induction of fungal cellulolytic enzymes using sugarcane bagasse and xylose-rich liquor as substrates. Braz. J. Chem. Eng. 37, 443–450 (2020). https://doi.org/10.1007/s43153-020-00055-5

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  • DOI: https://doi.org/10.1007/s43153-020-00055-5

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