The deiodinases regulate the activation and inactivation of Thyroid hormones (TH), in both physiological and pathological conditions. The three deiodinases, DIO1, DIO2 and DIO3, have different catalytic role and cellular and tissue distribution. Aim of this study is to evaluate a rat model of regional ischemia/reperfusion (I/R), the modification of cardiac main function after the administration of 6 µg/kg/day of triiodothyronine (T3), and the associated to DIO1, DIO2 and DIO3 gene expression. We also aim to study DIO1 and DIO2 protein levels in different left ventricular regions after an ischemic event. Four groups of rats were studied: sham-operated, sham-operated + T3, I/R rats and I/R rats + T3. DIO1, DIO2 and DIO3 expression were evaluated in I/R region (AAR: area-at-risk) and in a more distant region from ischemic wound (RZ: remote zone). In I/R group, circulating free-T3 (FT3) levels were significantly decreased with respect to basal values, whereas in I/R + T3 rats, FT3 levels were comparable to basal values. In AAR of I/R + T3 rats, DIO1 and DIO2 gene expression significantly increased with respect to sham. In RZ, DIO1 and DIO3 gene expression was significantly lower in sham and I/R rats when compared to I/R + T3. In sham + T3 group, DIO1 and DIO2 gene expression was not detectable, whereas DIO3 was significantly higher than in the other three groups. The present study gives interesting new insights on DIO1, DIO2 and DIO3 in the ischemic heart and their role in relation to T3-mediated amelioration of cardiac function and structure.

在生理和病理条件下，脱碘酶均调节甲状腺激素（TH）的活化和失活。DIO1，DIO2和DIO3这三种脱碘酶具有不同的催化作用以及细胞和组织的分布。这项研究的目的是评估大鼠局部缺血/再灌注（I / R）模型，给予6 µg / kg /天三碘甲状腺素（T3）后心脏主要功能的改变以及与DIO1，DIO2相关的模型和DIO3基因表达。我们还旨在研究缺血事件后左心室不同区域的DIO1和DIO2蛋白水平。研究了四组大鼠：假手术，假手术+ T3，I / R大鼠和I / R大鼠+ T3。在I / R区（AAR：有风险的区域）和距缺血性伤口较远的区域（RZ：偏远区）评估DIO1，DIO2和DIO3的表达。在I / R组中，循环游离T3（FT3）水平相对于基础值显着降低，而在I / R + T3大鼠中，FT3水平与基础值相当。在I / R + T3大鼠的AAR中，相对于假手术，DIO1和DIO2基因表达显着增加。在RZ中，与I / R + T3相比，假手术和I / R大鼠的DIO1和DIO3基因表达明显降低。在假手术+ T3组中，DIO1和DIO2基因的表达无法检测到，而DIO3则明显高于其他三组。本研究为缺血性心脏中的DIO1，DIO2和DIO3及其在T3介导的心脏功能和结构改善中的作用提供了有趣的新见解。FT3水平与基础值相当。在I / R + T3大鼠的AAR中，相对于假手术，DIO1和DIO2基因表达显着增加。在RZ中，与I / R + T3相比，假手术和I / R大鼠的DIO1和DIO3基因表达明显降低。在假手术+ T3组中，DIO1和DIO2基因的表达无法检测到，而DIO3则明显高于其他三组。本研究为缺血性心脏中的DIO1，DIO2和DIO3及其在T3介导的心脏功能和结构改善中的作用提供了有趣的新见解。FT3水平与基础值相当。在I / R + T3大鼠的AAR中，相对于假手术，DIO1和DIO2基因表达显着增加。在RZ中，与I / R + T3相比，假手术和I / R大鼠的DIO1和DIO3基因表达明显降低。在假手术+ T3组中，DIO1和DIO2基因的表达无法检测到，而DIO3则明显高于其他三组。本研究为缺血性心脏中的DIO1，DIO2和DIO3及其在T3介导的心脏功能和结构改善中的作用提供了有趣的新见解。DIO1和DIO2基因表达无法检测到，而DIO3则明显高于其他三组。本研究为缺血性心脏中的DIO1，DIO2和DIO3及其在T3介导的心脏功能和结构改善中的作用提供了有趣的新见解。DIO1和DIO2基因表达无法检测到，而DIO3则明显高于其他三组。本研究对缺血性心脏中的DIO1，DIO2和DIO3及其在T3介导的心脏功能和结构改善中的作用提供了有趣的新见解。