In vitro bioactivity and osteoblast cell viability studies of hydroxyapatite-incorporated silica aerogel,Journal of Sol-Gel Science and Technology

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In vitro bioactivity and osteoblast cell viability studies of hydroxyapatite-incorporated silica aerogel
Journal of Sol-Gel Science and Technology ( IF 2.3 ) Pub Date : 2020-08-11 , DOI: 10.1007/s10971-020-05386-w
Nor Suriani Sani , Nik Ahmad Nizam Nik Malek , Khairunadwa Jemon , Mohammed Rafiq Abdul Kadir , Halimaton Hamdan

Abstract

Bioactivity and osteoblastic growth of hydroxyapatite (HA)-incorporated silica aerogels (SA) (HAESA) with different ratio of HA/SiO₂ (0.1, 0.5, 1.0, and 1.3 HA/SiO₂) were investigated. Bioactivity of HAESA was studied by immersing all samples in simulated body fluids (SBF) for 7 and 14 days. The recovered samples were then characterised using Fourier transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy combined with energy dispersive X-ray analyser (FESEM–EDX) and the analysis of phosphate loss in the solution. These samples were also investigated for their effect on cell viability and proliferation abilities against normal human osteoblast cells in vitro after 24 and 48 h of exposure times. The HA/SiO₂ ratio affected its bioactivity in which the bioactivity increased to 0.5 (HAESA-0.5) and declined at higher ratios (1.0 and 1.3). A similar trend was observed for cell viability and proliferation assays. HAESA-0.5 (HA/SiO₂ ratio of 0.5), which possesses the optimal characteristics of SA and HA, resulted in the highest osteoblastic growth due to the synergistic effects between SA and HA. Thus, HAESA-0.5 could be an alternative biomaterial compared to silica- and HA-based biomaterials for bone implant application due to its higher bioactivity in SBF with the improved performance of osteoblast growth.

Hydroxyapatite-incorporated silica aerogel with different weight ratio of HA/SiO₂ (HAESA) was studied for their bioactivity in simulated body fluid and in vitro osteoblastic growth.

中文翻译：

掺入羟基磷灰石的二氧化硅气凝胶的体外生物活性和成骨细胞活力研究

摘要

研究了不同比例的HA / SiO ₂（0.1、0.5、1.0和1.3 HA / SiO ₂）的掺入羟基磷灰石（HA）的二氧化硅气凝胶（SA）（HAESA）的生物活性和成骨生长。通过将所有样品浸入模拟体液（SBF）中7天和14天来研究HAESA的生物活性。然后使用傅里叶变换红外光谱，X射线衍射，场发射扫描电子显微镜结合能量色散X射线分析仪（FESEM–EDX）对回收的样品进行表征，并分析溶液中的磷酸盐损失。还研究了这些样品在暴露24和48小时后对体外对正常人成骨细胞的细胞活力和增殖能力的影响。HA / SiO ₂比率影响其生物活性，其中生物活性增加至0.5（HAESA-0.5），并以较高比率（1.0和1.3）下降。在细胞活力和增殖测定中观察到类似趋势。具有SA和HA最佳特性的HAESA-0.5（HA / SiO ₂比率为0.5），由于SA和HA之间的协同作用，导致成骨细胞的生长最高。因此，与基于二氧化硅和基于HA的生物材料相比，HAESA-0.5可以替代生物材料，因为它在SBF中具有更高的生物活性，并且具有成骨细胞生长性能的提高，因此可用于骨植入物。

研究了不同重量比的HA / SiO ₂（HAESA）结合羟基磷灰石的二氧化硅气凝胶在模拟体液和体外成骨细胞生长中的生物活性。