The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed ‘actin chromobodies’ (ACs), targeted to organelle membranes to enable high-resolution imaging of sub-organellar actin dynamics.

中文翻译：

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肌动蛋白染色体成像揭示了亚细胞器肌动蛋白动力学。

肌动蛋白细胞骨架在细胞中发挥着多种关键作用，从细胞迁移到细胞器动力学。调节细胞器动力学的小而短暂的肌动蛋白结构很难用荧光显微镜检测到，因此很难确定肌动蛋白丝是否与特定的膜直接相关。为了解决这些限制，我们开发了荧光蛋白标记的肌动蛋白纳米抗体，称为“肌动蛋白染色体”（AC），靶向细胞器膜，以实现亚细胞器肌动蛋白动力学的高分辨率成像。