Cold atmospheric pressure plasma treatment is currently being explored as an alternative way to improve the germination and growing parameters of plant seeds. However, it is important to pay attention to the effect of plasma treatment on DNA damage of the seeds as well as detailed characteristics of plasma composition and parameters. The aim of this work was to study the DNA damage of plasma-treated pea seeds (Pisum sativum L.) and plasma parameters such as the chemical composition of plasma gaseous compounds and plasma radiation. Seeds were treated with plasma using the diffuse coplanar surface barrier discharge generated in different working gases (ambient air, nitrogen, oxygen and different mixtures of oxygen and nitrogen) at atmospheric pressure and at 60 s, 180 s and 300 s exposure times. DNA damage was studied using the single cell-gel electrophoresis called the comet assay and the plasma parameters were investigated by Fourier transform infrared spectroscopy and optical emission spectroscopy. Experiments in different ratios of oxygen and nitrogen were realized in order to understand the reaction mechanism between the ambient air plasma and the treated seeds. Based on our results, ambient air plasma appears to be the most advantageous for the plasma treatment due to no significant DNA damage because of the proper combination of plasma composition in combination with water vapor present in ambient air.

中文翻译：

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冷气压等离子体对豌豆种子的影响：幼苗的 DNA 损伤和等离子体的光学诊断

目前正在探索冷大气压等离子体处理作为改善植物种子发芽和生长参数的替代方法。然而，重要的是要注意等离子体处理对种子 DNA 损伤的影响以及血浆成分和参数的详细特征。这项工作的目的是研究等离子体处理的豌豆种子 (Pisum sativum L.) 的 DNA 损伤和等离子体参数，如等离子体气体化合物的化学成分和等离子体辐射。在大气压和 60 秒、180 秒和 300 秒的暴露时间下，使用在不同工作气体（环境空气、氮气、氧气和不同的氧气和氮气混合物）中产生的漫射共面表面阻挡放电对种子进行等离子体处理。使用称为彗星试验的单细胞凝胶电泳研究 DNA 损伤，并通过傅里叶变换红外光谱和光发射光谱研究等离子体参数。实现了不同氧氮比例的实验，以了解环境空气等离子体与处理过的种子之间的反应机理。根据我们的结果，由于等离子体成分与环境空气中存在的水蒸气的适当组合，环境空气等离子体似乎对等离子体处理最有利，因为没有显着的 DNA 损伤。实现了不同氧氮比例的实验，以了解环境空气等离子体与处理过的种子之间的反应机理。根据我们的结果，由于等离子体成分与环境空气中存在的水蒸气的适当组合，环境空气等离子体似乎对等离子体处理最有利，因为没有显着的 DNA 损伤。实现了不同氧氮比例的实验，以了解环境空气等离子体与处理过的种子之间的反应机理。根据我们的结果，由于等离子体成分与环境空气中存在的水蒸气的适当组合，环境空气等离子体似乎对等离子体处理最有利，因为没有显着的 DNA 损伤。