Purpose

The present study was focused on the synthesis and characterization of cerium with hydroxyapatite for biomedical applications.

Method

Hydroxyapatite (HAp) substituted with variable concentration (0.075, 0.150, 0.225%) of cerium (Ce) was synthesized by sol–gel method. The structural and morphological properties were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDAX). The average crystallite size of the synthesized samples was determined as 20 nm. The functional group has been identified by FTIR. Antibacterial and anti-inflammatory focused the antibacterial activity and albumin denaturation of cerium. Blood compatibility was performed by hemolytic assay.

Result

The structural property was characterized by X-ray diffraction and crystallite size was determined as 20 nm. The FTIR spectra confirmed the presence of hydroxyl and phosphate functional groups. Antibacterial studies revealed that all cerium-doped hydroxyapatite samples exhibit good antibacterial activity against the pathogens Streptococcus oralis and Streptococcus pyogenes. Hemolysis ratios of the cerium-doped hydroxyapatite sample indicate good blood compatibility. Anti-inflammatory results exhibit high inhibition percentage of albumin denaturation for cerium-doped hydroxyapatite samples.

Conclusion

This study suggests that the cerium proves that it is a good material for biomedical applications.

中文翻译：

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溶胶-凝胶法合成铈掺杂羟基磷灰石的生物医学体外研究

目的

本研究的重点是用于生物医学的羟基磷灰石铈的合成与表征。

方法

通过溶胶-凝胶法合成了浓度可变（0.075，0.150，0.225％）的铈（Ce）的羟基磷灰石（HAp）。通过X射线衍射（XRD），扫描电子显微镜（SEM）和能量色散X射线分析（EDAX）对结构和形态特性进行了表征。合成样品的平均微晶尺寸确定为20nm。FTIR已经确定了该官能团。抗菌和消炎集中了铈的抗菌活性和白蛋白变性。通过溶血测定进行血液相容性。

结果

通过X射线衍射表征结构性质，并将微晶尺寸确定为20nm。FTIR光谱证实了羟基和磷酸酯官能团的存在。抗菌研究表明，所有铈掺杂的羟基磷灰石样品均对口腔链球菌和化脓性链球菌病原体表现出良好的抗菌活性。铈掺杂的羟基磷灰石样品的溶血率表明其良好的血液相容性。抗炎结果显示，对于铈掺杂的羟基磷灰石样品，白蛋白变性具有很高的抑制百分比。

结论

这项研究表明，铈证明它是生物医学应用的良好材料。