Food safety and quality are issues of great concern to food producers and consumers. Key challenges towards achieving these include availability of rapid, user-friendly, economic and reliable techniques to detect problems such as antibiotic residues. Therefore, the present study was conducted to evaluate performance of plastic film test plate (PFTP) as influenced by antibiotic type, test bacterium, inoculum volume and concentration. Comparison was made to the microtiter test plate (MTP) and the National Standard method (SN/T 3979-2014). For this, antibiotic susceptibility testing was conducted on four bacterial types (Micrococcus luteus, Streptococcus thermophillus suspensions, Staphylococcus aureus and Coliform bacteria) against three antibiotics (penicillin G, sulfadiazine and tetracycline). Results showed high susceptibility of Micrococcus luteus to penicillin G with minimum inhibitory concentration of 3 µg L⁻¹ and 1 µg L⁻¹ via PFTP and MTP respectively. Optimum performance was realized at bacterial concentration of 10⁴ CFU ml⁻¹ with detection limit of 1 µg L⁻¹, sensitivity and predictive positive value (PPV) of 81–83% and 97–100% respectively. Detection time was recorded as 6 and 9 h for MTP and PFTP respectively compared to the 18–24 h of the National Standard method (SN/T 3979–2014). The microbiological plastic film test plate under optimized bacterial culture conditions demonstrated tremendous potential for rapid and reliable detection of antibiotics in milk.

食品安全和质量是食品生产者和消费者极为关注的问题。实现这些目标的关键挑战包括提供快速，用户友好，经济和可靠的技术来检测抗生素残留等问题。因此，本研究旨在评估受抗生素类型，测试细菌，接种量和浓度影响的塑料膜测试板（PFTP）的性能。与微量滴定板（MTP）和国家标准方法（SN / T 3979-2014）进行了比较。为此，对四种细菌类型（黄微球菌，热链球菌悬液，金黄色葡萄球菌和大肠菌）进行了抗生素敏感性测试。）针对三种抗生素（青霉素G，磺胺嘧啶和四环素）。结果显示，通过PFTP和MTP分别检测到，微球菌对青霉素G的敏感性很高，最低抑菌浓度分别为3 µg L ^-1和1 µg L ^-1。在细菌浓度为10 ⁴  CFU ml ^-1且检测限为1 µg L ^-1的条件下实现了最佳性能，敏感性和预测阳性值（PPV）分别为81–83％和97–100％。MTP和PFTP的检测时间分别记录为6和9 h，而国家标准方法（SN / T 3979-2014）的检测时间为18-24 h。在优化的细菌培养条件下，微生物塑料薄膜测试板证明了快速可靠检测牛奶中抗生素的巨大潜力。