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Optimization of microbiological plastic film test plate conditions for rapid detection of antibiotics in milk

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Abstract

Food safety and quality are issues of great concern to food producers and consumers. Key challenges towards achieving these include availability of rapid, user-friendly, economic and reliable techniques to detect problems such as antibiotic residues. Therefore, the present study was conducted to evaluate performance of plastic film test plate (PFTP) as influenced by antibiotic type, test bacterium, inoculum volume and concentration. Comparison was made to the microtiter test plate (MTP) and the National Standard method (SN/T 3979-2014). For this, antibiotic susceptibility testing was conducted on four bacterial types (Micrococcus luteus, Streptococcus thermophillus suspensions, Staphylococcus aureus and Coliform bacteria) against three antibiotics (penicillin G, sulfadiazine and tetracycline). Results showed high susceptibility of Micrococcus luteus to penicillin G with minimum inhibitory concentration of 3 µg L−1 and 1 µg L−1 via PFTP and MTP respectively. Optimum performance was realized at bacterial concentration of 104 CFU ml−1 with detection limit of 1 µg L−1, sensitivity and predictive positive value (PPV) of 81–83% and 97–100% respectively. Detection time was recorded as 6 and 9 h for MTP and PFTP respectively compared to the 18–24 h of the National Standard method (SN/T 3979–2014). The microbiological plastic film test plate under optimized bacterial culture conditions demonstrated tremendous potential for rapid and reliable detection of antibiotics in milk.

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Funding

We gratefully acknowledge the financial support from Jilin Provincial Science & Technology Development Plan under International Science and Technology Cooperation Project: Research on Rapid Detection Technology of Antibiotic Residues in Milk. (No.: 20200801072GH).

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Authors and Affiliations

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Moeketsi Ntakatsane, Ping Chen, Jingsheng Liu & Linlin Xu

  2. School of Life Sciences, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Katleho Senoko

  3. Faculty of Agriculture, National University of Lesotho, Roma 180, Maseru, Lesotho

    Moeketsi Ntakatsane, Poloko Mosebi & Katleho Senoko

Authors
  1. Moeketsi Ntakatsane
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  2. Ping Chen
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  3. Jingsheng Liu
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  4. Poloko Mosebi
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  5. Linlin Xu
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  6. Katleho Senoko
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Correspondence to Ping Chen.

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Ntakatsane, M., Chen, P., Liu, J. et al. Optimization of microbiological plastic film test plate conditions for rapid detection of antibiotics in milk. Food Measure 14, 3473–3482 (2020). https://doi.org/10.1007/s11694-020-00576-0

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  • DOI: https://doi.org/10.1007/s11694-020-00576-0

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