Orchidaceae is one of the largest plant families and includes species with high economic, ecological, and medicinal importance. Orchid seeds are produced in high amounts; however, these seeds show low germination percentage and have specific germination requirements. To enhance the germination and development of orchids, in vitro techniques have been successfully applied. Natural extracts can be used as a biostimulant in the culture medium, showing improved germination, elongation, and rooting. Thus, the main objective of this study was to determine the effects of Kappaphycus alvarezii aqueous extract in the in vitro development of Epidendrum secundum, evaluating its influence on plantlet morphoanatomy. The K. alvarezii extract was also characterized by nuclear magnetic resonance (NMR) spectroscopy. E. secundum plantlets obtained after the in vitro germination were subjected to in vitro elongation experiment in the presence of K. alvarezii extract (0, 6, 12, 25, 50, and 100 mg L^− 1). Our results indicated that the aqueous extract of K. alvarezii has a positive effect, stimulating rooting, plantlet development, fresh mass gain, and newly formed shoots of E. secundum plantlets, mainly at 50 mg L^− 1 concentration. The use of K. alvarezii extract indicates potential application in tissue culture and plant production, due to its biostimulating effect on plants. Considering the great market demand for new technologies and the growing interest in the application of marine resources, our results present relevant data on the biostimulating potential of this extract, which can be successfully applied for the cultivation of other species of orchids and species of ecological and economic interest.

兰科是最大的植物科之一，并且包括具有高度经济，生态和医学重要性的物种。兰花种子产量高；但是，这些种子发芽率低，并且有特定的发芽要求。为了增强兰花的发芽和发育，已成功应用了体外技术。天然提取物可以用作培养基中的生物刺激剂，表现出更好的发芽，伸长和生根。因此，主要目标本研究的是确定的效果卡帕藻中的体外发育含水提取物Epidendrum孔型，评价其上苗morphoanatomy影响。该卡帕藻提取物的特征还在于核磁共振波谱学。将体外发芽后获得的苏云金芽孢杆菌幼苗在阿尔瓦雷克斯氏菌提取物（0、6、12、25、50和100 mg L ^-1）的存在下进行体外延伸实验。我们的结果表明，K。alvarezii的水提取物具有积极的作用，主要在50 mg L ^-1浓度下刺激生根，幼苗生长，新鲜质量增加和苏云金芽苗的新芽。K. alvarezii的使用提取物由于其对植物的生物刺激作用，表明其在组织培养和植物生产中的潜在应用。考虑到对新技术的巨大市场需求以及对海洋资源应用的日益增长的兴趣，我们的研究结果提供了有关该提取物具有生物刺激潜力的相关数据，这些数据可以成功地用于其他兰花物种的种植以及生态和生物物种的种植。经济利益。