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Improved in vitro development of Epidendrum secundum (Orchidaceae) by using aqueous extract of the seaweed Kappaphycus alvarezii (Rhodophyta, Solieriaceae)

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Abstract

Orchidaceae is one of the largest plant families and includes species with high economic, ecological, and medicinal importance. Orchid seeds are produced in high amounts; however, these seeds show low germination percentage and have specific germination requirements. To enhance the germination and development of orchids, in vitro techniques have been successfully applied. Natural extracts can be used as a biostimulant in the culture medium, showing improved germination, elongation, and rooting. Thus, the main objective of this study was to determine the effects of Kappaphycus alvarezii aqueous extract in the in vitro development of Epidendrum secundum, evaluating its influence on plantlet morphoanatomy. The K. alvarezii extract was also characterized by nuclear magnetic resonance (NMR) spectroscopy. E. secundum plantlets obtained after the in vitro germination were subjected to in vitro elongation experiment in the presence of K. alvarezii extract (0, 6, 12, 25, 50, and 100 mg L− 1). Our results indicated that the aqueous extract of K. alvarezii has a positive effect, stimulating rooting, plantlet development, fresh mass gain, and newly formed shoots of E. secundum plantlets, mainly at 50 mg L− 1 concentration. The use of K. alvarezii extract indicates potential application in tissue culture and plant production, due to its biostimulating effect on plants. Considering the great market demand for new technologies and the growing interest in the application of marine resources, our results present relevant data on the biostimulating potential of this extract, which can be successfully applied for the cultivation of other species of orchids and species of ecological and economic interest.

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Acknowledgements

This work was supported by the Brazilian funding agencies CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). The authors are grateful to Dr. Leila Hayashi and Msc. Filipe A.S. Neves from Laboratório de Camarões Marinhos Seção de Macroalgas for kindly providing the Kappaphycus alvarezii extract used in this work.

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Authors and Affiliations

  1. Programa de Pós-graduação em Botânica, Departamento de Botânica, Universidade Federal do Paraná, Curitiba, PR, 81531-980, Brazil

    Julio César de Araújo Amatuzzi, Leila do Nascimento Vieira & Hugo Pacheco de Freitas Fraga

  2. Programa de Pós-graduação em Agronomia-Produção Vegetal, Universidade Federal do Paraná, Curitiba, PR, Brazil

    Bruno Francisco Sant’Anna-Santos

  3. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, 81531-980, Brazil

    Miguel Daniel Noseda

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  1. Julio César de Araújo Amatuzzi
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  2. Leila do Nascimento Vieira
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Correspondence to Hugo Pacheco de Freitas Fraga.

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de Araújo Amatuzzi, J.C., do Nascimento Vieira, L., Sant’Anna-Santos, B.F. et al. Improved in vitro development of Epidendrum secundum (Orchidaceae) by using aqueous extract of the seaweed Kappaphycus alvarezii (Rhodophyta, Solieriaceae). Acta Physiol Plant 42, 136 (2020). https://doi.org/10.1007/s11738-020-03129-6

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  • DOI: https://doi.org/10.1007/s11738-020-03129-6

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