The Neurospora crassa AOD1 protein is a mitochondrial alternative oxidase that passes electrons directly from ubiquinol to oxygen. The enzyme is encoded by the nuclear aod-1 gene and is produced when the standard electron transport chain is inhibited. We previously identified eleven strains in the N. crassa single gene deletion library that were severely deficient in their ability to produce AOD1 when grown in the presence of chloramphenicol, an inhibitor of mitochondrial translation that is known to induce the enzyme. Three mutants affected previously characterized genes. In this report we examined the remaining mutants and found that the deficiency of AOD1 was due to secondary mutations in all but two of the strains. One of the authentic mutants contained a deletion of the yvh1 gene and was found to have a deficiency of aod-1 transcripts. The YVH1 protein localized to the nucleus and a post mitochondrial pellet from the cytoplasm. A zinc binding domain in the protein was required for rescue of the AOD1 deficiency. In other organisms YVH1 is required for ribosome assembly and mutants have multiple phenotypes. Lack of YVH1 in N. crassa likely also affects ribosome assembly leading to phenotypes that include altered regulation of AOD1 production.

中文翻译：

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单一基因缺失突变体的表征影响神经孢菌中替代氧化酶生产：yvh1基因的作用。

的粗糙脉孢菌AOD1蛋白是直接从泛醇传递电子到氧线粒体交替氧化酶。该酶由aod-1核基因编码，在抑制标准电子传输链时产生。我们先前在克雷萨猪笼草中鉴定了11种菌株单个基因缺失文库，在存在氯霉素（已知可诱导该酶的线粒体翻译抑制剂）的条件下生长时，其产生AOD1的能力严重不足。三个突变体影响了先前表征的基因。在这份报告中，我们检查了其余的突变体，发现AOD1的缺乏是由于除两个菌株以外的所有菌株的二次突变所致。真实的突变体之一包含yvh1基因的缺失，并发现其缺乏aod-1成绩单。YVH1蛋白定位在细胞核和来自细胞质的线粒体沉淀后。蛋白质中的锌结合结构域是挽救AOD1缺陷所必需的。在其他生物中，YVH1是核糖体组装所必需的，突变体具有多种表型。猪笼草中缺乏YVH1也可能影响核糖体装配，导致表型改变，包括对AOD1产量的调节。