Chitosan-based glycerol-plasticized membranes were prepared aiming to develop low-cost and biocompatible material for wound dressing. They were produced by casting/solvent evaporation, and their formation mechanisms were attributed to intra- and inter-hydrogen bonds between materials. The results show that the glycerol acted as plasticizer and changed the proliferation of fibroblast cells on chitosan membranes. A high increase in the contact angle between water and membrane was observed comparing pure chitosan (more hydrophilic) and chitosan-glycerol (more hydrophobic) membranes. The angle values were found to be ~ 40 and ~ 130 degrees for chitosan and chitosan/glycerol 30% (w/w) membranes, respectively. In comparison with pure chitosan, glycerol-mixed membranes showed lower glass transition temperatures and are less brittle when handling. The antibacterial properties of such membranes against Gram-negative (E. coli) and Gram-positive (S. aureus and B. Cereus) microorganisms were tested, showing efficient growth inhibition. In vitro studies over fibroblast cells using plasticized membranes demonstrated good viability and enhanced cell proliferation in comparison with those composed of pure chitosan. Such results suggest that the as-prepared chitosan-glycerol membrane is a promising material for use as wound dressing in tissue engineering applications.

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基于壳聚糖的甘油增塑膜：杀菌和成纤维细胞生长特性

制备了基于壳聚糖的甘油增塑膜，旨在开发用于伤口敷料的低成本和生物相容性材料。它们是通过浇铸/溶剂蒸发产生的，它们的形成机制归因于材料之间的氢键和氢键。结果表明，甘油起到增塑剂的作用，改变了壳聚糖膜上成纤维细胞的增殖。与纯壳聚糖（更亲水）和壳聚糖-甘油（更疏水）膜相比，水和膜之间的接触角显着增加。发现壳聚糖和壳聚糖/甘油 30% (w/w) 膜的角度值分别为～40 度和～130 度。与纯壳聚糖相比，甘油混合膜显示出较低的玻璃化转变温度，并且在处理时不易碎。测试了这种膜对革兰氏阴性（大肠杆菌）和革兰氏阳性（金黄色葡萄球菌和蜡状芽孢杆菌）微生物的抗菌特性，显示出有效的生长抑制。使用塑化膜对成纤维细胞进行的体外研究表明，与由纯壳聚糖组成的细胞相比，该细胞具有良好的活力和增强的细胞增殖。这些结果表明，所制备的壳聚糖-甘油膜是一种很有前途的材料，可用作组织工程应用中的伤口敷料。使用塑化膜对成纤维细胞进行的体外研究表明，与由纯壳聚糖组成的细胞相比，该细胞具有良好的活力和增强的细胞增殖。这样的结果表明，所制备的壳聚糖-甘油膜是一种很有前途的材料，可用作组织工程应用中的伤口敷料。使用塑化膜对成纤维细胞进行的体外研究表明，与由纯壳聚糖组成的细胞相比，该细胞具有良好的活力和增强的细胞增殖。这些结果表明，所制备的壳聚糖-甘油膜是一种很有前途的材料，可用作组织工程应用中的伤口敷料。