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Chitosan-based glycerol-plasticized membranes: bactericidal and fibroblast cellular growth properties

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Abstract

Chitosan-based glycerol-plasticized membranes were prepared aiming to develop low-cost and biocompatible material for wound dressing. They were produced by casting/solvent evaporation, and their formation mechanisms were attributed to intra- and inter-hydrogen bonds between materials. The results show that the glycerol acted as plasticizer and changed the proliferation of fibroblast cells on chitosan membranes. A high increase in the contact angle between water and membrane was observed comparing pure chitosan (more hydrophilic) and chitosan-glycerol (more hydrophobic) membranes. The angle values were found to be ~ 40 and ~ 130 degrees for chitosan and chitosan/glycerol 30% (w/w) membranes, respectively. In comparison with pure chitosan, glycerol-mixed membranes showed lower glass transition temperatures and are less brittle when handling. The antibacterial properties of such membranes against Gram-negative (E. coli) and Gram-positive (S. aureus and B. Cereus) microorganisms were tested, showing efficient growth inhibition. In vitro studies over fibroblast cells using plasticized membranes demonstrated good viability and enhanced cell proliferation in comparison with those composed of pure chitosan. Such results suggest that the as-prepared chitosan-glycerol membrane is a promising material for use as wound dressing in tissue engineering applications.

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Acknowledgments

This study was partly financed by the Coordination for the Improvement in Higher Education Personnel – CAPES - Brazil (Finance Code 001), the São Paulo Research Foundation – FAPESP (Grant number: 2017/19470-8) and the National Council for Scientific and Technological Development – CNPq.

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Authors and Affiliations

  1. Scientific and Technological Institute, Brazil University, 235 Carolina Fonseca Street, Zip code 08230-030, São Paulo, SP, Brazil

    Joiciara Garcia Caroni, Cleber Ferraresi, Lívia Assis Garcia & Adriana Pavinatto

  2. Department of Chemical Engineering, Institute of Technological and Exact Sciences - ICTE, Federal University of Triângulo Mineiro (UFTM), 1250 Randolfo Borges Júnior Avenue, Zip code 38064-200, Uberaba, MG, Brazil

    Alexia Victoria de Almeida Mattos, Mônica Hitomi Okura, Ana Claudia Granato Malpass & Rafaela Cristina Sanfelice

  3. Department of Physiotherapy, Federal University of São Carlos (UFSCar), Washington Luiz Road, Zip code 13565-905, São Carlos, SP, Brazil

    Kelly Rossetti Fernandes

  4. São Carlos Institute of Physics, University of São Paulo (USP), 400 Trabalhador São Carlense Avenue, Zip code 13566-590, São Carlos, SP, Brazil

    Debora Terezia Balogh

  5. Department of Biosciences, Federal University of São Paulo (UNIFESP), 136 Silva Jardim Street, Zip code 11015-020, Santos, SP, Brazil

    Ana Cláudia Muniz Renno

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  1. Joiciara Garcia Caroni
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Caroni, J.G., de Almeida Mattos, A.V., Fernandes, K.R. et al. Chitosan-based glycerol-plasticized membranes: bactericidal and fibroblast cellular growth properties. Polym. Bull. 78, 4297–4312 (2021). https://doi.org/10.1007/s00289-020-03310-4

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