Blackleg is a devastating disease of cabbage (C genome), but so far, only a few resistant cabbage lines have been identified, and the resistance-related genes are yet to be identified. In contrast, numerous R loci have been mapped to the A or B genome. High ancestral synteny among Brassicaceae genomes suggest that homologous regions of R loci in the A or B genome may contain functional R gene(s) in the C genome. Here, we investigated the collinear region of a major Brassica napus R locus, LepR1′, in Brassica oleracea to identify genes of putative disease resistance class via comprehensive in silico analysis. The LepR1′ locus is collinear to a 17.29-Mbp region in chromosome C02 of Brassica oleracea and hosted a total of 1,392 genes, and 36 of these genes contained NBS, LRR, TIR, F-box and RLK domains. The expression of these 36 genes was profiled using qRT-PCR in the cotyledons of resistant (SCNU-03) and susceptible (SCNU-59) lines at 0, 6, 24, and 48 h after inoculation with Leptosphaeria maculans isolates 00–100s and 03–02s having the corresponding avirulent gene, AvrLep1. Among these genes, a cluster of 10 genes were differentially expressed. NBS-LRR gene Bo2g131620 had a 45-bp insertion/deletion (indel) mutation in the 3rd intron between resistant and susceptible lines. The higher expression in resistant lines against both isolates and the indel mutation in Bo2g131620 suggest that these genes may have roles in blackleg resistance in cabbage. Mapping and functional analysis will be necessary to validate such a role. Saturating the region with enough markers is recommended during mapping.

黑腿病是白菜（C基因组）的毁灭性疾病，但到目前为止，仅鉴定了少数抗性白菜系，并且尚未鉴定出与抗性相关的基因。相反，许多R基因座已被定位到A或B基因组。十字花科基因组之间的高祖先同源性表明，A或B基因组中R基因座的同源区域可能在C基因组中包含功能性R基因。在这里，我们调查的一个主要的共线区域油菜[R基因，LepR1“ ，在甘蓝通过全面的计算机分析来鉴定推定的抗病类的基因。该LepR1“该基因座与甘蓝型油菜的C02染色体上的17.29-Mbp区域共线，共有1,392个基因，其中36个基因包含NBS，LRR，TIR，F-box和RLK结构域。用qRT-PCR分别在接种黄腐杆菌（Leptosphaeria maculans）分离株00–100s和0,6,24和48 h后，在抗性（SCNU-03）和易感性（SCNU-59）子叶中使用qRT-PCR对这些基因进行了分析03-02具有相应的无毒基因AvrLep1。在这些基因中，有10个基因的簇被差异表达。NBS-LRR基因Bo2g131620在抗性和易感品系之间的第三个内含子中有一个45 bp的插入/缺失（indel）突变。Bo2g131620中针对分离物和indel突变的抗性系中较高的表达表明，这些基因可能与甘蓝的黑腿病抗性有关。映射和功能分析对于验证这样的角色将是必要的。建议在映射过程中用足够的标记使区域饱和。