The aim of this study is to investigate the effect of lncRNA DUXAP8 on proliferation and apoptosis of ovarian cancer cells, and to explore its potential mechanism. DUXAP8 interfering and overexpressing cell lines were constructed and the cell proliferation and apoptosis were tested. Hematoxylin–eosin, TdT-mediated dUTP nick end labeling, and immunohistochemistry were used to detect the effect of DUXAP8 on the ability of tumor formation. Quantitative real-time polymerase chain reaction and western blot were used to detect the mRNA and protein expression of miR-590-5p and YAP1, respectively. Dual luciferase assay was used to determine the target relationship between DUXAP8, miR-590-5p, and YAP1. DUXAP8 interference and miR-590-5p down-regulated cell lines were further constructed. Compared with normal ovarian cells, the expression of DUXAP8 in ovarian cancer cells was significantly increased, while the expression of miR-590-5p was decreased (p < 0.05). After DUXAP8 interference, cell proliferation and colony formation were decreased, and apoptosis was increased. The results of in vivo experiment are consistent with the in vitro experiments. The expression of miR-590-5p was up-regulated and the expression of YAP1 was decreased after DUXAP8 interference. Moreover, miR590-5p inhibitor can attenuate the effect of DUXAP8 interference on ovarian cancer cells. Taken together, lncRNA DUXAP8 can regulate the proliferation and apoptosis of ovarian cancer cells, and its mechanism may be related to the regulation of YAP1 gene by targeting miR-590-5p.

这项研究的目的是研究lncRNA DUXAP8对卵巢癌细胞增殖和凋亡的影响，并探讨其潜在机制。构建了DUXAP8干扰和过度表达的细胞系，并测试了细胞增殖和凋亡。苏木精-曙红，TdT介导的dUTP缺口末端标记和免疫组化方法用于检测DUXAP8对肿瘤形成能力的影响。实时定量聚合酶链反应和蛋白质印迹分别用于检测miR-590-5p和YAP1的mRNA和蛋白质表达。使用双重荧光素酶测定法确定DUXAP8，miR-590-5p和YAP1之间的靶标关系。进一步构建了DUXAP8干扰和miR-590-5p下调的细胞系。与正常卵巢细胞相比，p  ＜0.05）。DUXAP8干扰后，细胞增殖和集落形成减少，凋亡增加。体内实验的结果与体外实验一致。DUXAP8干扰后，miR-590-5p的表达上调，YAP1的表达降低。而且，miR590-5p抑制剂可以减弱DUXAP8干扰对卵巢癌细胞的作用。综上所述，lncRNA DUXAP8可以调节卵巢癌细胞的增殖和凋亡，其机制可能与通过靶向miR-590-5p调控YAP1基因有关。