Phylogenetic studies with Zika virus (ZIKV) have been conducted in Brazil. In this study, we sequenced 8 new sequences of the ZIKV envelope (E) gene from strains of cases from the Paraná and Mato Grosso do Sul states in 2016. A low phylogenetic signal was observed, with more than 40% of unresolved quartets, and the Maximum Likelihood Tree grouped all sequences in the Brazilian branches within the Asian genotype. In addition, a Shannon entropy analysis was conducted, showing a high stability in the E protein through the ZIKV polyprotein. Taken together, these results suggest a high degree of conservation in the ZIKV E gene from the recent American outbreaks.

中文翻译：

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对美国疫情中寨卡病毒包膜基因保护的见解

巴西已对寨卡病毒 (ZIKV) 进行了系统发育研究。在这项研究中，我们对 2016 年巴拉那州和南马托格罗索州病例毒株的 ZIKV 包膜 (E) 基因的 8 个新序列进行了测序。观察到系统发育信号较低，超过 40% 的四联体未解析，并且最大似然树将巴西分支中亚洲基因型的所有序列分组。此外，进行了香农熵分析，显示 E 蛋白通过 ZIKV 多蛋白具有高稳定性。总而言之，这些结果表明最近美国爆发的 ZIKV E 基因具有高度保守性。