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Insights on Zika virus envelope gene conservation in American outbreaks

  • Bacterial Fungal and Virus Molecular Biology - Short Communication
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Abstract

Phylogenetic studies with Zika virus (ZIKV) have been conducted in Brazil. In this study, we sequenced 8 new sequences of the ZIKV envelope (E) gene from strains of cases from the Paraná and Mato Grosso do Sul states in 2016. A low phylogenetic signal was observed, with more than 40% of unresolved quartets, and the Maximum Likelihood Tree grouped all sequences in the Brazilian branches within the Asian genotype. In addition, a Shannon entropy analysis was conducted, showing a high stability in the E protein through the ZIKV polyprotein. Taken together, these results suggest a high degree of conservation in the ZIKV E gene from the recent American outbreaks.

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Acknowledgments

We thank the Laboratório Central do Estado do Paraná, Brazil, for supplying the samples used in this study.

Funding

This work was supported by Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná, Brazil (Grant. no. 040/2016).

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Authors and Affiliations

  1. Laboratório de Virologia Clínica, Universidade Estadual de Maringá – UEM, Avenida Colombo, 5790, Maringá, Paraná, 87020-900, Brazil

    Mateus V. Thomazella, Quirino A. Lima Neto, Francisco F. Duarte Junior, Fernando A. Jorge, Déborah de Castro Moreira, Amauri D. Leal Junior & Dennis A. Bertolini

  2. Laboratório Central do Estado do Paraná – LACEN-PR, Curitiba, Paraná, Brazil

    Mayra M. Presibella & Irina N. Riediger

  3. Instituto Butantan, São Paulo, São Paulo, Brazil

    Rafael A. da Silva & Isabel Maria V. G. de Carvalho

  4. Universidade Federal de São Paulo - UNIFESP/EPM, São Paulo, São Paulo, Brazil

    Rafael A. da Silva & Isabel Maria V. G. de Carvalho

Authors
  1. Mateus V. Thomazella
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  2. Quirino A. Lima Neto
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  3. Francisco F. Duarte Junior
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  4. Fernando A. Jorge
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  5. Déborah de Castro Moreira
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  6. Amauri D. Leal Junior
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  9. Rafael A. da Silva
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  10. Isabel Maria V. G. de Carvalho
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  11. Dennis A. Bertolini
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Corresponding author

Correspondence to Mateus V. Thomazella.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

Ethical approval

The use of human samples was approved by the Permanent Research Ethics Committee of the State University of Maringá (Protocol no. 1.751.311).

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Responsible Editor: Mauricio Nogueira.

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Thomazella, M.V., Lima Neto, Q.A., Duarte Junior, F.F. et al. Insights on Zika virus envelope gene conservation in American outbreaks. Braz J Microbiol 51, 1601–1605 (2020). https://doi.org/10.1007/s42770-020-00349-3

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  • DOI: https://doi.org/10.1007/s42770-020-00349-3

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