Anti-apoptotic gene Ced-9 enhanced resistance against Fusarium oxysporum f. sp. cubense (Foc) in the susceptible banana cultivar Rasthali by arresting tissue necrosis. The embryogenic cell suspension of banana cultivar Rasthali was stably transformed with Ced-9 gene and transformed lines were regenerated independently. The putative transgenic lines were analyzed with PCR using gene primers and further subjected to Southern blot to estimate copy number. The root-challenge bioassay with Foc showed 17–51% Vascular Discoloration Index in independent transformants compared to untransformed banana cv Rasthali (98% VDI). Four transgenic events showed a higher level of resistance over a period of 6 months. Overcoming tissue necrosis is the most ideal method to avoid Fusarium multiplication and spread in banana. Oxidative stress-induced cell necrosis is prevented by the activation of antiapoptotic pathways by Ced-9 and is proving to be an effective method to control this dreaded disease. This is the first report from India on the generation of transgenic banana cultivar Rasthali expressing antiapoptotic Ced-9 gene for resistance to Fusarium wilt.

抗凋亡基因 Ced-9 增强对尖孢镰刀菌的抗性。sp. 立方体(Foc) 在易感香蕉品种 Rasthali 中通过阻止组织坏死。用Ced-9基因稳定转化香蕉品种Rasthali的胚性细胞悬液并独立再生转化株系。使用基因引物通过PCR分析推定的转基因品系，并进一步进行Southern印迹以估计拷贝数。与未转化的香蕉 cv Rasthali (98% VDI) 相比，使用 Foc 的根攻击生物测定显示独立转化体的血管变色指数为 17-51%。在 6 个月的时间里，四个转基因事件显示出更高水平的抗性。克服组织坏死是避免镰刀菌在香蕉中繁殖和传播的最理想方法。通过 Ced-9 激活抗细胞凋亡途径可以防止氧化应激诱导的细胞坏死，并且被证明是控制这种可怕疾病的有效方法。这是来自印度的第一份关于转基因香蕉品种 Rasthali 的报告，该品种表达抗凋亡 Ced-9 基因以抵抗枯萎病。