Abstract
Anti-apoptotic gene Ced-9 enhanced resistance against Fusarium oxysporum f. sp. cubense (Foc) in the susceptible banana cultivar Rasthali by arresting tissue necrosis. The embryogenic cell suspension of banana cultivar Rasthali was stably transformed with Ced-9 gene and transformed lines were regenerated independently. The putative transgenic lines were analyzed with PCR using gene primers and further subjected to Southern blot to estimate copy number. The root-challenge bioassay with Foc showed 17–51% Vascular Discoloration Index in independent transformants compared to untransformed banana cv Rasthali (98% VDI). Four transgenic events showed a higher level of resistance over a period of 6 months. Overcoming tissue necrosis is the most ideal method to avoid Fusarium multiplication and spread in banana. Oxidative stress-induced cell necrosis is prevented by the activation of antiapoptotic pathways by Ced-9 and is proving to be an effective method to control this dreaded disease. This is the first report from India on the generation of transgenic banana cultivar Rasthali expressing antiapoptotic Ced-9 gene for resistance to Fusarium wilt.
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Acknowledgements
The authors are thankful to DBT-BIRAC for funding under the project Development and Transfer of Technology from Queensland University of Technology (QUT), Australia to India for Biofortification and Disease Resistance in Banana. This is the part of Ph.D. dissertation work of first author.
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This study was funded by the Department of Biotechnology—Biotechnology Industry Research Association Council.
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Conceived and designed the experiments: TRU, HDS and CS. Performed the experiments: CS and HDS Analyzed the data: CS, TRU, HDS, MU and SS. Paper: CS, TRU, HDS and HRG
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Authors Mrs. Sunisha. C, Dr. Sowmya H.D, Dr. Usharani T.R, Mr. Umesha M, Dr.Sriram.S and Gopalkrishna H.R have received research grants from the Department of Biotechnology—Biotechnology Industry Research Association Council.
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Sunisha, C., Sowmya, H.D., Usharani, T.R. et al. Induction of Ced9 mediated anti-apoptosis in commercial banana cultivar Rasthali for stable resistance against Fusarium wilt. 3 Biotech 10, 371 (2020). https://doi.org/10.1007/s13205-020-02357-4
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DOI: https://doi.org/10.1007/s13205-020-02357-4