Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.,Food & Function

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Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata.
Food & Function ( IF 5.1 ) Pub Date : 2020-08-03 , DOI: 10.1039/d0fo01234c
Kai Qian ₁ , Ting Tan , Hui Ouyang , Shi-Lin Yang , Wei-Feng Zhu , Rong-Hua Liu , Quan Wen , Yu-Lin Feng

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A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification. The average molecular weight of PLP-1 was 16.2 kDa. Monosaccharide composition analysis showed that PLP-1 was composed of glucose as a glucan. The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-D-Glcp(1→ and →4)-β-D-Glcp(1→ with a molar ratio of 7.0 : 1.0. Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells. The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells. Therefore, PLP-1 could serve as a dietary supplement to ameliorate insulin resistance for diabetic patients.

中文翻译：

从葛根的根具有低血糖活性的同多糖的结构表征。

通过DEAE纤维素和Sephadex G-200凝胶色谱法纯化，从葛根的根部获得水溶性中性均多糖（PLP-1）。PLP-1的平均分子量为16.2kDa。单糖组成分析表明，PLP-1由葡萄糖组成的葡聚糖。在广泛的物理和化学分析的基础上表征了PLP-1的结构，这表明PLP-1的骨架主要由→3）-α - D- Glc p（1→和→4）-β组成- d -Glc p（1→的摩尔比为7.0：1.0。此外，还通过棕榈酸和高葡萄糖诱导的胰岛素抵抗性HepG2细胞研究了PLP-1的降血糖活性。结果表明，PLP-1可以通过增加PLP-1降低血糖浓度。调节PI3K和AKT的表达，下调FoxO1，PCK2和G6Pase在胰岛素抵抗细胞中的表达，因此，PLP-1可以作为改善糖尿病患者胰岛素抵抗的饮食补充。

更新日期：2020-08-19

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