Issue 8, 2020
From the journal:

Food & Function

Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata†

Kai Qian,‡ab   Ting Tan, ORCID logo ‡ab   Hui Ouyang,ab   Shi-Lin Yang,c   Wei-Feng Zhu,a   Rong-Hua Liu,a   Quan Wen ORCID logo *ab  and  Yu-Lin Feng*abc  

* Corresponding authors

a Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, Jiangxi, PR China
E-mail: qwen12@fudan.edu.cn

b The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, Nanchang 330006, Jiangxi, PR China
E-mail: fengyulin@126.com

c State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang 330006, Jiangxi, PR China

Abstract

A water-soluble neutral homopolysaccharide (PLP-1) was obtained from the roots of Pueraria lobata by DEAE cellulose and Sephadex G-200 gel chromatography purification. The average molecular weight of PLP-1 was 16.2 kDa. Monosaccharide composition analysis showed that PLP-1 was composed of glucose as a glucan. The structure of PLP-1 was characterized on the basis of extensive physical and chemical analysis, which indicated that the backbone of PLP-1 was mainly composed of →3)-α-D-Glcp(1→ and →4)-β-D-Glcp(1→ with a molar ratio of 7.0 : 1.0. Moreover, the hypoglycemic activity of PLP-1 was investigated by palmitic acid and high glucose induced insulin resistant HepG2 cells. The results elucidated that PLP-1 could decrease the glucose concentration by up-regulating the expression of PI3K and AKT, and down-regulating the expression of FoxO1, PCK2, and G6Pase in insulin resistant cells. Therefore, PLP-1 could serve as a dietary supplement to ameliorate insulin resistance for diabetic patients.

Graphical abstract: Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata

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Article information

DOI
https://doi.org/10.1039/D0FO01234C
Article type
Paper
Submitted
12 May 2020
Accepted
20 Jul 2020
First published
03 Aug 2020
This article is Open Access
Creative Commons BY-NC license

Food Funct., 2020,11, 7104-7114

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Structural characterization of a homopolysaccharide with hypoglycemic activity from the roots of Pueraria lobata

K. Qian, T. Tan, H. Ouyang, S. Yang, W. Zhu, R. Liu, Q. Wen and Y. Feng, Food Funct., 2020, 11, 7104 DOI: 10.1039/D0FO01234C

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