Abstract

A protectant for hypothermic preservation of human sperm based on glass-forming water–salt systems of vital metals (magnesium, zinc, and calcium) was developed for the first time. The method of sperm preservation without preliminary washing of seminal plasma is proposed, which retains the sperm viability and sperm motility parameters comparable to the values of native sperm. The proposed protectant and method of preparing human sperm for hypothermic storage at the first stage help to slow down the metabolism as evidenced by the complete loss of sperm motility and maintenance of the nearly 100% viability of the studied sperm. Once the protectant was removed, sperm motility is restored up to 90–100% compared to the indices of native sperm.

中文翻译：

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使用玻璃水盐系统制备人类精子以低温保存精液

摘要

首次开发了一种用于低温保存人类精子的保护剂，该保护剂基于玻璃形成的水-重要金属（镁，锌和钙）的盐体系。提出了一种无需预先清洗精浆即可保存精子的方法，该方法可保留与天然精子相当的精子活力和精子活力参数。拟议的保护剂和制备用于第一阶段低温保存的精子的方法有助于减缓新陈代谢，这是精子活力完全丧失和所研究精子近100％活力的维持所证明的。去除保护剂后，与天然精子指标相比，精子活力可恢复高达90–100％。