Abstract
A protectant for hypothermic preservation of human sperm based on glass-forming water–salt systems of vital metals (magnesium, zinc, and calcium) was developed for the first time. The method of sperm preservation without preliminary washing of seminal plasma is proposed, which retains the sperm viability and sperm motility parameters comparable to the values of native sperm. The proposed protectant and method of preparing human sperm for hypothermic storage at the first stage help to slow down the metabolism as evidenced by the complete loss of sperm motility and maintenance of the nearly 100% viability of the studied sperm. Once the protectant was removed, sperm motility is restored up to 90–100% compared to the indices of native sperm.
Similar content being viewed by others
REFERENCES
A. A. Kostyaev, A. K. Martusevich, and A. A. Andreev, Nauch. Obozr. Med. Nauki, No. 6, 54 (2016).
L. L. Kuleshova, J. M. Shaw, and A. O. Trouson, Cryobiology 43, 21 (2001).
H. Kanno, K. Kajwara, and K. Miyta, J. Chem. Phys. 132, 1945 (2010).
C. J. Hunt, Transfusion Medicine and Hemotherapy 46 (3), 134 (2019).
I. V. Arutinyan, S. O. Strokova, A. V. Makarov, et al., Kletoch. Tekhnol. Biol. Med., No. 3, 180 (2018).
S. O. Strokova, I. V. Arutinyan, S. M. Mullabaeva, et al., Akusher. Ginekol., No. 12, 5 (2018).
O. N. Sineva, T. D. Ivankova, and L. P. Terekhova, Antibiot. Khimioter., Nos. 3–4, 3 (2019).
K. Saito, Y. Kinoshita, and H. Kanno, Fertil. Steril. 65, 1210 (1996).
H. Kanno, K. Saito, and T. Ogawa, Fertil. Steril. 69, 127 (1998). https://doi.org/10.1016/S0015-0282(97)00439-1
J. M. Riel, T. T. Huang, and M. A. Ward, Arch. Androl. 53, 275 (2007).
J. M. Riel, Y. Yamauchi, and T. T. Huang, Biol. Reproduc. 85, 536 (2011). https://doi.org/10.1095/biolreprod.111.091322
M. Morisawa and M. Yoshda, Reproduct. Med. Biol., No. 4, 101 (2005).
D. A. Isaev, V. V. Zaeva, R. V. Bakuradze, et al., Probl. Reprod., No. 5, 33 (2009).
D. A. Isaev, E. E. Zakharova, I. V. Kapralova, et al., Probl. Reprod., No. 4, 65 (2015). https://doi.org/10.17116/repro201521465-70
I. V. Cherkashina, Candidate Dissertation in Biology (Khar’kov, 2007).
A. A. Odintsov, I. N. Kuchkov, I. V. Cherkashina, et al., Sovrem. Tekhnol. Med. 3, 47 (2011).
I. A. Kirilenko, Water–Electrolyte Glass-Forming Systems (Krasand, Moscow, 2016) [in Russian].
I. A. Kirilenko, Russ. J. Inorg. Chem. 62, 1819 (2017). https://doi.org/10.1134/S00360236171140042
I. A. Kirilenko, Russ. J. Inorg. Chem. 63, 1728 (2018). https://doi.org/10.1134/S0036023618130053
L. I. Kirilenko, L. I. Demina, and V. P. Danilov, Russ. J. Inorg. Chem. 64, 1282 (2019). https://doi.org/10.1134/S0036023619100073
WHO Laboratory Manual for the Examination and Processing of Human Semen (World Health Organization, Dep. Reproductive Health and Res, 2010; Kapital Print, Moscow, 2012).
Funding
This study was supported by the Presidium of Russian Academy of Sciences through Basic Research Program No. 37 “The Foundations for Designing Metallic, Ceramic, and Composite Structural Materials Characterized by Improved Performance Characteristics” and Basic Research Program No. 39 “The Foundations and Energy-Efficient Resource-Saving Innovative Technologies of Mineral Raw Material Processing and Disposal of Industrial and Household Waste.”
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflicts of interest.
Additional information
Translated by D. Terpilovskaya
Rights and permissions
About this article
Cite this article
Kirilenko, I.A., Vinokurov, A.A., Danilov, V.P. et al. Use of Glass-Forming Water–Salt Systems for Preparing Human Sperm to Hypothermic Semen Preservation. Russ. J. Inorg. Chem. 65, 989–991 (2020). https://doi.org/10.1134/S003602362006008X
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S003602362006008X