Vanda tessellata (Roxb.) Hook. ex G.Don (grey orchid) is a horticultural and medicinally potent, and conservation prioritized orchid species. The present study investigates asymbiotic seed germination, callus induction, somatic embryogenesis, encapsulation of somatic embryos (SEs), and retrieval of plantlets of V. tessellata from the artificial seeds after short-term cold storage at − 4 °C for 12 months. Seeds cultured on full strength Murashige and Skoog’s (MS) medium containing 2.0 mg L⁻¹ 6-benzylaminopurine (BAP) had the highest frequency of asymbiotic seed germination (94%) as compared with half- and one-fourth strength MS media supplemented with various concentrations and combinations of 6-furfurylaminopurine (kinetin) and BAP. The protocorm like bodies (PLBs) gained dedifferentiation and proliferated into embryogenic calli on MS medium containing 2.0 mg L⁻¹ BAP and 0.5 mg L⁻¹ indole-3 acetic acid (IAA). Somatic embryos (SEs) were differentiated from the callus when cultured on MS medium in combination with 1.0 mg L⁻¹ BAP and 0.5 mg L⁻¹ IAA. The morpho-anatomical elucidations authenticated somatic embryogenesis and various developmental stages of SEs from embryogenic calli. Short-term cold storage of SEs was carried out by encapsulating with 2% sodium alginate and 100 mM calcium chloride and stored under dark at − 4 °C for 12 months to conserve this valuable germplasm. The stored artificial seeds were germinated (91%) on MS medium incorporated with additives and 0.5 mg L⁻¹ each of BAP, kinetin, and IAA. Complete retrieval of plantlets was achieved in 8 week with formation of shoots (4.2 cm average length) and roots (2.5 cm). The well developed plantlets were acclimatized in the greenhouse with 90% survival rate.

万达·特塞拉塔（Roxb。）ex G.Don（灰色兰花）是一种园艺性强且具有药用价值且具有保护优先性的兰花品种。本研究调查非共生种子发芽，愈伤组织诱导，体细胞胚胎发生，体细胞胚的封装（SES），和小植株的检索V. tessellata从短期冷储存后的人工种子在- 4℃下12个月。在含有2.0 mg L ^-1的完全强度Murashige和Skoog（MS）培养基上培养的种子与补充了各种浓度和6-糠基氨基嘌呤（肌动蛋白）和BAP组合的强度为一半和四分之一的MS培养基相比，6-苄基氨基嘌呤（BAP）的非共生种子发芽频率最高（94％）。在含有2.0 mg L ^-1 BAP和0.5 mg L ^-1 indole-3乙酸（IAA）的MS培养基上，原球茎样体（PLBs）获得去分化并增殖为胚性愈伤组织。当在MS培养基上与1.0 mg L ^-1 BAP和0.5 mg L ^-1结合使用时，体细胞胚（SEs）从愈伤组织中分化出来IAA。形态解剖学鉴定证实了胚性愈伤组织的体细胞胚胎发生和SEs的不同发​​育阶段。通过用2％海藻酸钠和100 mM氯化钙包封进行SE的短期冷藏，并在−4°C的黑暗条件下保存12个月，以保存这种有价值的种质。储存的人造种子在掺有添加剂和BAP，激动素和IAA各自为0.5 mg L ^-1的MS培养基上发芽（91％）。在8周内完全恢复了幼苗，形成了芽（平均长度4.2厘米）和根（2.5厘米）。发育良好的小植株在温室中适应，成活率为90％。