Phyllosphere microorganisms are sensitive to fluctuations in wind, temperature, solar radiation, and rain. However, recent explorations of patterns in phyllosphere communities across time often focus on seasonal shifts and leaf senescence without measuring the contribution of environmental drivers and leaf traits. Here, we focus on the effects of rain on the phyllosphere bacterial community of the wetland macrophyte broadleaf cattail (Typha latifolia) across an entire year, specifically targeting days before and 1, 3, and 5 days after rain events. To isolate the contribution of precipitation from other factors, we covered a subset of plants to shield them from rainfall. We used targeted Illumina sequencing of the V4 region of the bacterial 16S rRNA gene to characterize phyllosphere community composition. Rain events did not have a detectable effect on phyllosphere community richness or evenness regardless of whether the leaves were covered from rain or not, suggesting that foliar microbial communities are robust to such disturbances. While climatic and leaf-based variables effectively modeled seasonal trends in phyllosphere diversity and composition, they provided more limited explanatory value at shorter time scales. These findings underscore the dominance of long-term seasonal patterns related to climatic variation as the main factor influencing the phyllosphere community.

气圈微生物对风，温度，太阳辐射和雨水的波动敏感。但是，近期对整个叶层群落模式的探索通常集中在季节性变化和叶片衰老，而没有测量环境驱动因素和叶片性状的贡献。在这里，我们集中于雨水对湿地大型植物阔叶香蒲（香蒲）的叶环细菌群落的影响），专门针对降雨事件发生之前，之后1、3和5天。为了将降水的贡献与其他因素隔离开来，我们覆盖了一部分植物以使其免受降雨的影响。我们使用细菌16S rRNA基因V4区域的靶向Illumina测序来表征叶球群落组成。不论叶片是否被雨水覆盖，降雨都没有对叶球群落的丰富度或均匀度产生可检测的影响，这表明叶面微生物群落对此类干扰具有抵抗力。尽管基于气候和基于叶的变量有效地模拟了叶圈多样性和组成的季节性趋势，但它们在较短的时间尺度上提供的解释价值有限。