Key Message

A novel and major QTL for the effective tiller number was identified on chromosomal arm 1BL and validated in two genetic backgrounds

Abstract

The effective tiller number (ETN) substantially influences plant architecture and the wheat yield improvement. In this study, we constructed a genetic map of the 2SY (20828/SY95-71) recombinant inbred line population based on the Wheat 55K array as well as the simple sequence repeat (SSR) and Kompetitive Allele Specific PCR (KASP) markers. A comparison between the genetic and physical maps indicated the marker positions were consistent in the two maps. Additionally, we identified seven tillering-related quantitative trait locus (QTLs), including Qetn-sau-1B.1, which is a major QTL localized to a 6.17-cM interval flanked by markers AX-89635557 and AX-111544678 on chromosome 1BL. The Qetn-sau-1B.1 QTL was detected in eight environments and explained 12.12–55.71% of the phenotypic variance. Three genes associated with the ETN were detected in the physical interval of Qetn-sau-1B.1. We used a tightly linked KASP marker, KASP-AX-110129912, to further validate this QTL in two other populations with different genetic backgrounds. The results indicated that Qetn-sau-1B.1 significantly increased the ETN by up to 23.5%. The results of this study will be useful for the precise mapping and cloning of Qetn-sau-1B.1.

中文翻译：

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面包小麦染色体臂 1BL 上有效分蘖数的新的、主要的和经过验证的 QTL。

关键信息

在染色体臂 1BL 上鉴定了一个新的和主要的有效分蘖数 QTL，并在两个遗传背景中得到验证

抽象的

有效分蘖数（ETN）显着影响植物结构和小麦产量的提高。在这项研究中，我们基于小麦 55K 阵列以及简单序列重复 (SSR) 和竞争等位基因特异性 PCR (KASP) 标记构建了 2SY (20828/SY95-71) 重组自交系群体的遗传图谱。遗传图和物理图之间的比较表明两个图中的标记位置是一致的。此外，我们鉴定了 7 个与分蘖相关的数量性状基因座 (QTL)，包括Qetn-sau-1B.1，它是定位于 6.17-cM 区间的主要 QTL，两侧为染色体 1BL 上的标记AX-89635557和AX-111544678 。Qetn -sau-1B.1在八种环境中检测到 QTL，并解释了 12.12-55.71% 的表型变异。在Qetn-sau-1B.1的物理区间中检测到三个与 ETN 相关的基因。我们使用紧密连锁的 KASP 标记KASP-AX-110129912来进一步验证其他两个具有不同遗传背景的群体中的 QTL。结果表明，Qetn-sau-1B.1显着增加了 ETN 高达 23.5%。这项研究的结果将有助于Qetn-sau-1B.1的精确定位和克隆。