Abstract
Key Message
A novel and major QTL for the effective tiller number was identified on chromosomal arm 1BL and validated in two genetic backgrounds
Abstract
The effective tiller number (ETN) substantially influences plant architecture and the wheat yield improvement. In this study, we constructed a genetic map of the 2SY (20828/SY95-71) recombinant inbred line population based on the Wheat 55K array as well as the simple sequence repeat (SSR) and Kompetitive Allele Specific PCR (KASP) markers. A comparison between the genetic and physical maps indicated the marker positions were consistent in the two maps. Additionally, we identified seven tillering-related quantitative trait locus (QTLs), including Qetn-sau-1B.1, which is a major QTL localized to a 6.17-cM interval flanked by markers AX-89635557 and AX-111544678 on chromosome 1BL. The Qetn-sau-1B.1 QTL was detected in eight environments and explained 12.12–55.71% of the phenotypic variance. Three genes associated with the ETN were detected in the physical interval of Qetn-sau-1B.1. We used a tightly linked KASP marker, KASP-AX-110129912, to further validate this QTL in two other populations with different genetic backgrounds. The results indicated that Qetn-sau-1B.1 significantly increased the ETN by up to 23.5%. The results of this study will be useful for the precise mapping and cloning of Qetn-sau-1B.1.
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Acknowledgements
This work is supported by the projects from the National Natural Science Foundation of China (31971937 and 31970243), the Key Research and Development Program of Sichuan Province (2018NZDZX0002), the Applied Basic Research Programs of Science and Technology Department of Sichuan Province (2020YJ0140), and the Key Projects of Scientific and Technological Activities for Overseas Students of Sichuan Province. We thank Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac) for editing the English text of a draft of this manuscript. We thank the anonymous referees for critical reading and revising this manuscript.
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JJL performed the research and drafted this manuscript. HPT and XRQ participated in carrying out the research and revising this manuscript. HL, CL, YT, SQL, AH, YM, SFD, and MD participated in phenotype measurement, field work, and data analysis. QTJ and YXL assisted with data collection and analysis. GYC, JRW, GDC, WL, and YFJ performed data processing and QTL analysis. YMW and XJL discussed results and revised the manuscript. YLZ guided the entire study, discussed results and revised this manuscript. JM designed the experiments, guided the entire study, participated in data analysis, and revised this manuscript. All authors participated in the research and approved the final manuscript.
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All experiments and data analyses were conducted in Sichuan. All authors contributed to the study and approved the final version for submission. The manuscript has not been submitted to any other journal.
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Jiajun Liu, Huaping Tang, and Xiangru Qu have contributed equally to this work.
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11103_2020_1035_MOESM12_ESM.tif
Supplementary file 12 Fig. S1 Synteny between genetic and physical positions of the mapped markers. Gen1A to Gen7D stand for the whole genome genetic maps of common wheat which were released in this study; Phy1A to Phy7D represent the physical maps for the 21 wheat chromosomes which were constructed by assigning 9030 SNPs to the wheat genome assembly using SNP flanking sequences as the query. Only one randomly marker is shown in this figure. (TIF 42974 kb)
11103_2020_1035_MOESM13_ESM.tif
Supplementary file13 Fig. S2 Phenotypic distribution of effective tiller number in different environments. Black arrow represents parent 20828 and red arrow represents the other parent SY95-71. (TIF 7959 kb)
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Supplementary file 14 Fig. S3 Correlation coefficients for effective tiller number (ETN) with plant height (PH), anthesis date (AD), 1,000-grain weight (TGW), spikelet number per spike (SNS) and yield per unit area (Yield). *Significance at the 0.05 probability level. (TIF 5089 kb)
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Supplementary file 15 Fig. S4 The 2-LOD confidence interval (CI) of five identified effective tiller number (ETN) QTL in all the test environments. a: the 2-LOD CI of Qetn-sau-1B.1; b: the 2-LOD CI of Qetn-sau-4A; c: the 2-LOD CI of Qetn-sau-6A; d: the 2-LOD CI of Qetn-sau-7A.1; e: the 2-LOD CI of Qetn-sau-7A.2. (TIF 3319 kb)
11103_2020_1035_MOESM16_ESM.tif
Supplementary file 16 Fig. S5 Expression patterns of three tillering-related genes in wheat. The expression values were retrieved from http://www.wheat-expression.com/ (Ricardo Ramirez-Gonzalez, et al. Science 2018 (361) 6403: eaar6089; Philippa et al. Plant Physiology 2016: pp.15.01667v1). (TIF 5089 kb)
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Liu, J., Tang, H., Qu, X. et al. A novel, major, and validated QTL for the effective tiller number located on chromosome arm 1BL in bread wheat. Plant Mol Biol 104, 173–185 (2020). https://doi.org/10.1007/s11103-020-01035-6
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DOI: https://doi.org/10.1007/s11103-020-01035-6