Purpose

The active ingredient recovery from the vegetable is a very attractive research field for the development of a sustainable economy; to revalue the jujube fruit (Zizyphus lotus) polysaccharide (ZLPS), an optimized green microwave-assisted method was used for the recovery and enrichment of the antioxidants present in a distilled water extract.

Methods

A series of 17 experiments including microwave power, irradiation time, and liquid-to-solid ratio independent parameters was designed by the response surface methodology to optimize the recovery of the polysaccharide extract.

Results

The optimal conditions were as follows: 600 W, 40 min, and 26.69 mL/g. Under these conditions, the experimental extraction yield was 13.98 ± 1.55% which is very close with the predicted value (14.08%), and this demonstrated the validation of the extraction model proposed. The polysaccharide extract exhibited a significant scavenging activity against ABTS^.+ (70.45%), DPPH*^.(66.02%), and FRAP (A = 0.63) with a very important anti-inflammatory activity using a protein denaturation method that showed a maximum inhibition of 95.33% at 200 μg/mL. Additionally, the membrane stabilization method showed a significant action and protection of human red blood cells (85.76%) in hypotonic-induced lysis solution and 86.45% in heat-induced lysis solution.

Conclusion

This study demonstrated the possibility of exploiting the microwave process to obtain extracts remarkably enriched with invaluable antioxidants from the jujube matrix. The operation time is short, and the antioxidant and anti-inflammatory activities of the distilled water extract were preserved.

中文翻译：

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微波辅助提取阿尔及利亚枣果肉和果皮多糖的响应面法优化

目的

从蔬菜中回收有效成分是发展可持续经济的一个非常有吸引力的研究领域。重估枣果（枣莲）多糖（ZLPS）中，使用用于在蒸馏水提取物回收和抗氧化剂存在的富集的优化绿色微波辅助方法。

方法

通过响应面方法设计了一系列17个实验，包括微波功率，辐照时间和液固比独立参数，以优化多糖提取物的回收率。

结果

最佳条件如下：600 W，40分钟和26.69 mL / g。在这种条件下，实验提取率为13.98±1.55％，与预测值（14.08％）非常接近，这证明了所提出的提取模型的有效性。多糖提取物表现出对ABTS ⁺（70.45％），DPPH *的明显清除活性^。（66.02％）和具有非常重要的抗炎活性的FRAP（A = 0.63），使用蛋白质变性方法在200μg/ mL时显示最大抑制95.33％。另外，膜稳定方法在低渗诱导的裂解液中显示出显着的作用并保护人红细胞（85.76％），在热诱导裂解液中显示出86.45％。

结论

这项研究证明了利用微波工艺从枣基质中获得富含宝贵抗氧化剂的提取物的可能性。操作时间短，并且保留了蒸馏水提取物的抗氧化和抗炎活性。