Abstract

A gram-positive gabapentin-degrading bacteria strain N.ISM.1 was isolated from the pharmaceutical plant soil. According to the analysis of 16S rRNA gene sequence, the strain was categorized as a Micrococcus luteus. Though the strain N.ISM.1 is capable of degrading gabapentin (GABA), this compound cannot provide enough carbon source for the isolated strain. M. luteus N.ISM.1 degrades GABA in the presence of glucose. The greater the concentration of secondary simpler carbon source (glucose) for cometabolism, the greater the biodegradation of GABA is. Around 97% of GABA was degraded within a course of 40 days after using 150 mg/L glucose as cometabolic simpler carbon source. M. luteus N.ISM.1 is a promising microorganism for the biodegradation of an anticonvulsant drug GABA contaminated environment and with the aid of cometabolic activity of glucose (150 mg/L) the degradation achieved perfection. GABA biodegradation metabolites were identified by a liquid chromatography-electrospray ionization tandem mass spectrometry. This is the very first report of GABA biodegradation as per our concern.

中文翻译：

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葡萄糖代谢对革兰氏阳性细菌黄球菌N.ISM.1对加巴喷丁（抗惊厥药）生物降解的影响。

摘要

从药用植物土壤中分离出革兰氏阳性加巴喷丁降解细菌菌株N.ISM.1。根据对16S rRNA基因序列的分析，该菌株被分类为黄微球菌。尽管菌株N.ISM.1能够降解加巴喷丁（GABA），但该化合物无法为分离出的菌株提供足够的碳源。黄曲霉N.ISM.1在葡萄糖存在下降解GABA。用于代谢的次要简单碳源（葡萄糖）的浓度越高，GABA的生物降解能力就越大。使用150 mg / L葡萄糖作为可代谢的简单碳源后的40天内，约有97％的GABA降解。黄麻N.ISM.1是一种有前途的微生物，可用于抗惊厥药GABA污染环境的生物降解，并借助葡萄糖的代谢活性（150 mg / L）达到理想的降解效果。通过液相色谱-电喷雾串联质谱法鉴定了GABA生物降解代谢产物。根据我们的关注，这是GABA生物降解的第一份报告。