Spring viremia of carp (SVC) is highly contagious and lethal disease in cyprinid fish, in particular common carps (Cyprinus carpio), causing numerous economic losses to the aquaculture industry. SVC is presently endemic disease in Europe, America, and several countries in Asia and its causative agent is spring viremia of carp virus (SVCV). In this study, a chitosan-alginate microcapsule probiotic vaccine expressing G protein of SVCV was prepared, and the immunogenicity in carps of orally administrated with the microcapsule probiotic vaccine was evaluated. Our results showed that the microcapsule probiotic vaccine can induce potent antigen-specific immune responses in carps via oral vaccination, and provide effective anti-SVCV protection for carps. Significantly, the microcapsule probiotic vaccine is suitable for mass fish immunization, suggesting a promising vaccine strategy for fish.

鲤鱼春季病毒血症（SVC）是高度传染性和致死性疾病，特别是鲤鱼（鲤鱼）），给水产养殖业造成许多经济损失。SVC目前是欧洲，美洲和亚洲几个国家的地方病，其病原是鲤鱼病毒的春季病毒血症（SVCV）。在这项研究中，制备了表达SVCV G蛋白的壳聚糖-海藻酸钠微胶囊益生菌疫苗，并评价了口服微胶囊益生菌疫苗对鲤鱼的免疫原性。我们的结果表明，微胶囊益生菌疫苗可以通过口服疫苗在鲤鱼中诱导有效的抗原特异性免疫反应，并为鲤鱼提供有效的抗SVCV保护。值得注意的是，微胶囊益生菌疫苗适用于鱼类的大规模免疫接种，这提示了一种有前途的鱼类疫苗策略。