The present study was aimed to extract tannins from methanolic extracts of Salix alba and determination of their antimicrobial potential. Antibacterial activity of tannins extracted from S. alba leaves and stem bark methanolic extracts was determined by using agar well diffusion assay against seven Gram-positive and Gram-negative bacterial strains including Streptococcus pyogenes, Staphylococcus aureus (1), S. aureus (2), Shigella sonnei, Escherichia coli (1), E. coli (2), and Neisseria gonorrhoeae, and zone of inhibition was measured in terms of millimeter. The highest inhibition zone was recorded at 17.33 ± 1.15 by using bark tannins (100 mg/ml) against S. aureus (2) after an incubation period of 48 h while no activity was reported against S. sonnei by using leaves tannins. It was observed that the tannins from the bark of the S. alba were more effective to kill all bacterial pathogens and the inhibition percentage increased with increasing the concentration of the tannins. Major classes of secondary metabolites were determined qualitatively and quantitatively. Leaves fraction of S. alba methanolic extract was enriched with Tannins, Quinones, Steroids, Resins, and Phenols while stem bark extracts were embellished with Flavonoids, Tannins, Quinones, and Phenols. Findings from this study will help to develop formulation from S. alba tannins and endorse the ethnopharmacological utilization of the evaluated plant.

本研究旨在从白柳的甲醇提取物中提取丹宁酸，并确定其抗菌潜力。通过琼脂孔扩散测定法测定了从白叶芥和茎皮甲醇提取物中提取的单宁酸对七种革兰氏阳性和革兰氏阴性细菌菌株（包括化脓性链球菌，金黄色葡萄球菌（1），金黄色葡萄球菌（2））的抗菌活性。，志贺氏志贺氏菌，大肠杆菌（1），大肠杆菌（2）和淋病奈瑟氏球菌，其抑制区以毫米为单位。使用树皮单宁（100 mg / ml）时，最高抑制区记录为17.33±1.15孵育48小时后，金黄色葡萄球菌（2）没有发现通过使用单宁叶对S. sonnei的活性。观察到，来自S. alba树皮的单宁更有效地杀死所有细菌病原体，并且抑制百分率随单宁浓度的增加而增加。次要代谢产物的主要类别是定性和定量确定的。苜蓿链球菌甲醇提取物的叶子部分富含单宁，醌，类固醇，树脂和苯酚，而茎皮提取物则装饰有黄酮，单宁，醌和苯酚。这项研究的结果将有助于开发白芥的配方 单宁酸，并支持被评估植物的民族药理学利用。