Using honey as a model sample, the aim of this study is to develop and validate a simple, rapid screening tool that allows for the visualization of constituents that contribute to the antioxidant activity of a complex natural product and to quantify their individual antioxidant effects even if their chemical identity is unknown. The method employs a validated analysis, which is based on the separation of constituents using high-performance thin-layer chromatography (HPTLC) followed by their visualization using DPPH* (2,2-diphenyl-1-picrylhydrazyl) as derivatizing reagent and the quantification of the antioxidant activity of individual bands expressed as gallic acid equivalents.

中文翻译：

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HPTLC-DPPH测定方法的开发和验证及其在蜂蜜分析中的应用

以蜂蜜为模型样品，本研究的目的是开发和验证一种简单，快速的筛选工具，该工具可以可视化对复杂天然产品的抗氧化活性有贡献的成分，并量化其单独的抗氧化作用，即使其化学身份未知。该方法采用了经过验证的分析，该分析基于使用高效薄层色谱（HPTLC）分离成分，然后使用DPPH *（2,2-二苯基-1-吡啶并肼基）作为衍生试剂并将其可视化的方法没食子酸根的抗氧化活性的单位表示为没食子酸当量。