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Branched architecture of fucoidan characterized by dynamic and static light scattering
Colloid and Polymer Science ( IF 2.2 ) Pub Date : 2020-07-23 , DOI: 10.1007/s00396-020-04706-4
Ekaterina R. Gasilova , Irina M. Lapina , Anna A. Kulminskaya , Yury A. Skorik

Branched architecture of fucoidans (sulfated fucose-rich polysaccharides) gained considerable interest in recent years in view of the effect of this branching on fucoidan’s biological activity. The aim of this work was to characterize the branching architecture of the pristine and hydrolyzed fucoidan macromolecules of the marine seaweed Fucus vesiculosus. We studied the influence of the duration of acidic hydrolysis on the fucoidan macromolecules. The structure-sensitive ratios of macromolecular radii, obtained by dynamic and static (SLS) light scattering, and capillary viscometry corresponded to spherical form of the macromolecules, indicating their branched architecture. The structure-sensitive ratios were analyzed with respect to the molecular weight distributions obtained by size-exclusion chromatography. The Kratky representation of the angular dependences of SLS intensity provided a spectacular evidence for the fucoidans’ branching. For the first time, the fucoidans’ branching was characterized quantitatively by fitting the scattering form factor developed for hyperbranched macromolecules. Under acidic hydrolysis, the molecular mass and size of the fucoidan macromolecules decreased, but the globular form of macromolecules remained unchanged. Upon increasing the time of the acidic hydrolysis, the ζ-potential of the fucoidans changed from − 14 to − 27 mV. The hydrolyzed fucoidans, in turn, exhibited pronounced polyelectrolyte expansion in aqueous solutions when compared with the unhydrolyzed one. Graphical abstract Graphical abstract

中文翻译:

以动态和静态光散射为特征的褐藻糖胶的分支结构

鉴于这种分支对岩藻依聚糖生物活性的影响,岩藻依聚糖(富含硫酸化岩藻糖的多糖)的分支结构近年来引起了极大的兴趣。这项工作的目的是表征海洋海藻 Fucus vesiculosus 的原始和水解岩藻依聚糖大分子的分支结构。我们研究了酸性水解时间对岩藻多糖大分子的影响。通过动态和静态 (SLS) 光散射和毛细管粘度测定法获得的大分子半径的结构敏感比率对应于大分子的球形,表明它们的分支结构。相对于通过尺寸排阻色谱法获得的分子量分布分析结构敏感比率。SLS 强度的角度依赖性的 Kratky 表示为岩藻依聚糖的分支提供了壮观的证据。首次通过拟合为超支化大分子开发的散射形状因子来定量表征岩藻依聚糖的支化。在酸性水解作用下,岩藻多糖大分子的分子量和尺寸减小,但大分子的球状形式保持不变。随着酸性水解时间的增加,岩藻依聚糖的 ζ 电位从 - 14 mV 变为 - 27 mV。与未水解的相比,水解的岩藻依聚糖在水溶液中表现出明显的聚电解质膨胀。图形摘要图形摘要 通过拟合为超支化大分子开发的散射形状因子来定量表征岩藻依聚糖的支化。在酸性水解作用下,岩藻多糖大分子的分子量和尺寸减小,但大分子的球状形式保持不变。随着酸性水解时间的增加,岩藻依聚糖的 ζ 电位从 - 14 mV 变为 - 27 mV。与未水解的相比,水解的岩藻依聚糖在水溶液中表现出明显的聚电解质膨胀。图形摘要图形摘要 通过拟合为超支化大分子开发的散射形状因子来定量表征岩藻依聚糖的支化。在酸性水解作用下,岩藻多糖大分子的分子量和尺寸减小,但大分子的球状形式保持不变。随着酸性水解时间的增加,岩藻依聚糖的 ζ 电位从 - 14 mV 变为 - 27 mV。与未水解的相比,水解的岩藻依聚糖在水溶液中表现出明显的聚电解质膨胀。图形摘要图形摘要 但大分子的球状形式保持不变。随着酸性水解时间的增加,岩藻依聚糖的 ζ 电位从 - 14 mV 变为 - 27 mV。与未水解的相比,水解的岩藻依聚糖在水溶液中表现出明显的聚电解质膨胀。图形摘要图形摘要 但大分子的球状形式保持不变。随着酸性水解时间的增加,岩藻依聚糖的 ζ 电位从 - 14 mV 变为 - 27 mV。与未水解的相比,水解的岩藻依聚糖在水溶液中表现出明显的聚电解质膨胀。图形摘要图形摘要
更新日期:2020-07-23
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