Abstract

Bisphenol S (BPS) is a major alternative to Bisphenol A (BPA) newly applied in food packing material and disrupts reproductive and developmental endpoints through endocrine pathways. This study aimed to develop a rapid, sensitive, and accurate chemiluminescence immunoassay (CLIA) for the detection of BPS in canned beverage. Anti-BPS monoclonal antibody (mAb) was generated by immunizing Balb/c mice with immunogen (BPS–BSA conjugation). CLIA assay conditions, including 3-[9-(((3-(N-succinimidyloxycarboxypropyl) [4-methxylphenyl] sulfonyl) amine) carboxyl]-10-acridiniumyl)-1-propanesulfonate inner salt (NSP-SA-NHS) labeling ratio (1:40), antigen concentration (1 μg mL⁻¹) and antibody titer (1:8000; 125 ng mL⁻¹ in PBS (0.01 M, pH 7.4)) were determined, respectively. The half inhibition concentration (IC₅₀), detection limit (IC₁₀), and working range of the CLIA in canned beverage were 0.04, 1.48, and 0.32–5 ng mL⁻¹, respectively. Cross-reactivity with other bisphenols (4,4′-thiodiphenol, Bisphenol F, 4,4′- dihydroxy benzophenone, Bisphenol A and Bisphenol B) was less than 0.56%, and the recovery rates of BPS in canned beverage ranged from 80 to 109.2% (intra-assay) and from 82 to 108.4% (inter-assay). The results of CLIA were compared with high-performance liquid chromatography–UV (HPLC–UV) analysis, showing a good correlation of 0.956. After proper sample pre-treatment, this method allows relatively large amounts of sample analysis in a limited time period (30 min). Thus, it was deemed suitable and efficient for routine screening of BPS in canned beverage.

Graphic abstract

中文翻译：

---

化学发光免疫分析方法，使用NSP-SA标记的特异性单克隆抗体定量罐装饮料中的双酚S

摘要

双酚S（BPS）是食品包装材料中新应用的双酚A（BPA）的主要替代品，它通过内分泌途径破坏生殖和发育终点。这项研究旨在开发一种快速，灵敏和准确的化学发光免疫分析法（CLIA），用于检测罐装饮料中的BPS。抗BPS单克隆抗体（mAb）是通过用免疫原（BPS-BSA偶联）免疫Balb / c小鼠而产生的。CLIA分析条件，包括3- [9-（（（（3-（N-琥珀酰亚胺氧羰基丙基）[4-甲基苯基苯基]磺酰基）胺]羧基] -10-ac基）-1-丙烷磺酸内酯（NSP-SA-NHS）标记比例（1:40），抗原浓度（1μgmL ^-1）和抗体滴度（1：8000; 125 ng mL ^-1分别测定在PBS（0.01 M，pH 7.4）中的浓度。罐装饮料中CLIA的半数抑制浓度（IC ₅₀），检出限（IC ₁₀）和工作范围为0.04、1.48和0.32–5 ng mL ^-1， 分别。与其他双酚（4,4'-硫代双酚，双酚F，4,4'-二羟基二苯甲酮，双酚A和双酚B）的交叉反应小于0.56％，罐装饮料中BPS的回收率范围为80至109.2％（批内）和82％至108.4％（批间）。CLIA的结果与高效液相色谱-UV（HPLC-UV）分析进行了比较，相关系数为0.956。经过适当的样品预处理后，此方法可在有限的时间段（30分钟）内进行相对大量的样品分析。因此，它被认为适合常规检测罐装饮料中的BPS。

图形摘要