Abstract
Bisphenol S (BPS) is a major alternative to Bisphenol A (BPA) newly applied in food packing material and disrupts reproductive and developmental endpoints through endocrine pathways. This study aimed to develop a rapid, sensitive, and accurate chemiluminescence immunoassay (CLIA) for the detection of BPS in canned beverage. Anti-BPS monoclonal antibody (mAb) was generated by immunizing Balb/c mice with immunogen (BPS–BSA conjugation). CLIA assay conditions, including 3-[9-(((3-(N-succinimidyloxycarboxypropyl) [4-methxylphenyl] sulfonyl) amine) carboxyl]-10-acridiniumyl)-1-propanesulfonate inner salt (NSP-SA-NHS) labeling ratio (1:40), antigen concentration (1 μg mL−1) and antibody titer (1:8000; 125 ng mL−1 in PBS (0.01 M, pH 7.4)) were determined, respectively. The half inhibition concentration (IC50), detection limit (IC10), and working range of the CLIA in canned beverage were 0.04, 1.48, and 0.32–5 ng mL−1, respectively. Cross-reactivity with other bisphenols (4,4′-thiodiphenol, Bisphenol F, 4,4′- dihydroxy benzophenone, Bisphenol A and Bisphenol B) was less than 0.56%, and the recovery rates of BPS in canned beverage ranged from 80 to 109.2% (intra-assay) and from 82 to 108.4% (inter-assay). The results of CLIA were compared with high-performance liquid chromatography–UV (HPLC–UV) analysis, showing a good correlation of 0.956. After proper sample pre-treatment, this method allows relatively large amounts of sample analysis in a limited time period (30 min). Thus, it was deemed suitable and efficient for routine screening of BPS in canned beverage.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant Numbers 31572556; 31873006); the Key Program for International S&T Cooperation Project of Shaanxi Province (Grant Number 2017KW-ZD-10); the Incubation Project on State Key Laboratory of Biological Resources and Ecological Environment of Qinba Areas (Grant Number SLGPT2019KF04-04); and the FCT I.P, the Ministério da Ciência Tecnologia e Ensino Superior (MCTES), the European Regional Development Fund (ERDF) (Grant Number POCI-01-0145-FEDER-007569).
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XZ conceived and designed the study. FFY and LX conducted experiments, analyzed data and prepared the manuscript. CC and XL analyzed data. XZ and ACPD critically revised the manuscript. All authors discussed results, edited and commented on the manuscript.
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All animal experimental protocols were reviewed and approved by the Ethics Committee of Shaanxi University of Technology for the use of Laboratory Animals (2019–008, Chinese-German Joint Laboratory for Natural Product Research).
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Yang, F., Xu, L., Liu, X. et al. Chemiluminescence immunoassay approach to quantify Bisphenol S in canned beverage using a NSP-SA-labeled specific monoclonal antibody. Eur Food Res Technol 246, 1857–1865 (2020). https://doi.org/10.1007/s00217-020-03539-3
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DOI: https://doi.org/10.1007/s00217-020-03539-3