A new wheat–Leymus mollis (2n = 4x = 28, NsNsXmXm) disomic addition line M11003-3-1-12-10 (M10) was developed and selected from the BC1F5 progeny of a cross between wheat cv. 7182 and octoploid Tritileymus M47 (2n = 8x = 56, AABBDDNsNs). Cytological observations showed that M10 contained 44 chromosomes and formed 22 bivalents at meiotic metaphase I. The sequential fluorescence in situ hybridization–genomic in situ hybridization (FISH–GISH) analysis using the oligonucleotide Oligo-pSc119.2, Oligo-pTa535 and L. mollis genomic DNA as probes revealed that M10 carried a pair of L. mollis chromosomes and a whole set of 42 wheat chromosomes. Two EST–STS markers and six PLUG (PCR-based Landmark Unique Gene) markers confirmed that the added L. mollis chromosomes in M10 belonged to homoeologous group 7 and were designated as Lm#7Ns, so M10 was deduced to be a wheat–L. mollis Lm#7Ns disomic addition line. Field disease evaluation demonstrated that M10 showed a high level of resistance to stripe rust at the adult stage and the resistance could be ultimately traced to the L. mollis. Consequently, the introduced Lm#7Ns chromosomes were responsible for the stripe rust resistance. The novel disomic addition line M10 could be exploited as an important genetic resource for wheat resistance breeding.

中文翻译：

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抗条锈病小麦-羊草Lm#7Ns二体加成系的开发与表征

开发了一种新的小麦-羊草 (2n = 4x = 28, NsNsXmXm) 二体加成系 M11003-3-1-12-10 (M10)，并从小麦品种之间杂交的 BC1F5 后代中筛选出来。7182 和八倍体小麦 M47（2n = 8x = 56，AABBDDNsNs）。细胞学观察表明 M10 包含 44 条染色体，并在减数分裂中期 I 形成 22 个二价体。 使用寡核苷酸 Oligo-pSc119.2、Oligo-pTa535 和 L. mollis 进行的顺序荧光原位杂交 - 基因组原位杂交 (FISH-GISH) 分析作为探针的基因组DNA显示M10携带一对L. mollis染色体和一整套42条小麦染色体。2个EST-STS标记和6个PLUG（PCR-based Landmark Unique Gene）标记证实M10中添加的L. mollis染色体属于同源组7，命名为Lm#7Ns，因此 M10 被推断为小麦-L。mollis Lm#7Ns 二体加成线。田间病害评估表明，M10 在成虫阶段表现出高水平的条锈病抗性，这种抗性最终可以追溯到 L. mollis。因此，引入的 Lm#7Ns 染色体是抗条锈病的原因。新型二体加成系M10可作为小麦抗性育种的重要遗传资源。